荧光猝灭法对头孢羟氨苄和头孢拉定荷移反应研究  

Study of Cefadroxil and Cephradine Charge Transfer Process by Fluorescence Quenching Method

作　　者：刘一鸣[1] 宣春生[1] 李文英[1] 冯杰[1] 

机构地区：[1]煤科学与技术教育部和山西省重点实验室太原理工大学,山西太原030024

出　　处：《光谱学与光谱分析》2009年第2期441-445,共5页Spectroscopy and Spectral Analysis

基　　金：教育部创新团队发展计划资助(IRT0517);教育部新世纪优秀人才支持计划(NCET-05-267;NCET-06-261)资助

摘　　要：研究了头孢羟氨苄和头孢拉定对荧光素的荧光猝灭反应及相互作用机理。因形成荷移络合物,头孢羟氨苄和头孢拉定对荧光素的荧光强度有很强的猝灭作用。采用紫外-可见光谱、红外光谱结合量子化学计算分析了荷移反应过程,揭示了头孢菌素类药物的成键规律和反应机理。在此基础上建立了测定头孢羟氨苄和头孢拉定含量的荧光光谱法。络合物的激发波长和发射波长分别为483,517和519 nm,头孢羟氨苄和头孢拉定浓度分别在0.3-13.5 mg.L^-1和0.1-1.2 mg.L^-1范围内为线性关系,相关系数分别是r=0.9997和r=0.9993。应用该方法测定了头孢羟氨苄和头孢拉定药物制剂含量,回收率分别在99.63%-99.91%和99.71%-100.08%之间。测定结果与文献值基本吻合。A simple and sensitive fluorescence quenching method was developed for the examination of cefadroxil and eephradine. The method is based on the reaction of fluorescein with cefadroxil or cephradine, resulting in the formation of complexes with weak fluorescence, i.e. quenching the fluorescence intensity of the system. By the use of absorption spectroscopy, infrared spectroscopy and quantum chemistry calculation the reaction process of the cephalosporins and the bonding of reaction mechanism were basically analyzed. Based on this, the fluorescence spectrometry method for the determination of the contents of cefadroxil and cephradine was developed： The maximum excitation and emission wavelength was at 483 nm, and 517 nm and 519 nm respectively. The linear calibration curves were obtained over the cefadroxil and cephradine concentration ranges of 0. 3-13. 5 mg · L^- 1 and 0. 1-1.2 mg · L^-1 respectively The proposed method has been successfully applied to the examination of the subject drugs in its pharmaceutical dosage with good precision and accuracy. The recoveries are 99. 63%-99. 91% and 99.71%-100. 08% respectively.

关 键 词：头孢羟氨苄 头孢拉定 荧光素 荧光猝灭法 荷移反应 

分 类 号：O657.3[理学—分析化学]