辐射损伤后造血细胞增殖能力的变化  

THE EFFECT OF RADIATION INJURY ON THE PROLIFERATION CAPACITY OF BONE MARROW HEMATOPOIETIC CELLS IN VIVO

作　　者：邹仲敏[1] 罗成基[1] 郭朝华[1] 施炎[1] 陈兆珍[2] 李才安[2] 

机构地区：[1]第三军医大学防原医学教研室 [2]第三军医大学中心实验室

出　　处：《辐射研究与辐射工艺学报》1998年第1期34-37,共4页Journal of Radiation Research and Radiation Processing

基　　金：军队"九五"指令性课题

摘　　要：辐射损伤后，存活的造血细胞的增殖能力决定着伤后造血功能的状态和预后。本实验以辐射损伤小鼠为模型，用ＢｒｄＵ掺入法和ＣＦＵＧＭ培养等方法，观察到４～１０Ｇｙ射线全身照射后４ｈ，骨髓细胞的增殖指数就显著下降，在观察时间内一直处于较低水平（仅为对照组的２０％或更低）。在４～１０Ｇｙ照射后４ｈ或更长，几乎看不到骨髓造血细胞的ＣＦＵＧＭ集落，推测存在一个“成集落休克期”。可见，射线在损伤造血细胞的同时，还明显抑制存活细胞的增殖能力，这可能是某些增强细胞增殖能力的药物能促进伤后造血功能恢复的基础之一。The recovery of hematopoiesis post radiation injury depends on the proliferation ability of survived bone marrow hematopoietic cells (BMHCs). In this experiment the proliferation ability of BMHCs of 4～10 Gy γ irradiated mice was studied with the methods of BrdU incorperation and CFU GM culture. The proliferation index(PI) declined significantly at 4h post injury and kept at this low level( 20% of control) with 48h. The CFU GM could hardly be detected at 4h after 4～10 Gy radiation, hinting that there might be a phase of colonizing shock early after radiation injury. The data suggest that the proliferation ability of BMHCs is inhibited by irradiation except its direct killing effect, and this might be the basis for drugs of stimulating cell proliferation to accelerate hematopoiesis recovery after radiation injury.

关 键 词：辐射损伤 造血细胞 细胞增殖 骨髓 造血功能 

分 类 号：R818.02[医药卫生—放射医学] R818.83[医药卫生—临床医学]