水稻C端结合蛋白家族基因OsCtBP-A的克隆及表达分析  

Analysis on Cloning and Expression of OsCtBP-A,a C Terminal Binding Protein(CtBPs) Family Gene of Rice

作　　者：李磊[1] 吴茂森[1] 李广旭[1] 何晨阳[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国农业科技导报》2009年第1期86-91,共6页Journal of Agricultural Science and Technology

基　　金：中央财政国家重点实验室自主研究课题专项(SKL2007SR06)资助

摘　　要：利用5′-RACE方法克隆了在水稻与白叶枯病菌(Xanthomonas oryzaepv.oryzae,简称Xoo)互作过程中下调表达的水稻C端结合蛋白家族基因OsCtBP-A的全长序列。通过生物信息学方法分析了该基因及编码蛋白质的结构特征,OsCtBP-A由424氨基酸组成,N端具有2-Hacid_dh_C保守结构域,C端具有植物CtBPs家族特有的NLS和PEST基序,氨基酸序列与拟南芥和牵牛花CtBPs同源物的同源性为63%;基因启动子区推测具有ABA和SA应答调控元件。OsCtBP-A基因对不同信号分子应答的RTQ-PCR分析表明,水杨酸(SA)、茉莉酸甲酯(MeJA)、乙烯(ETH)和脱落酸(ABA)处理水稻后均能诱导该基因的转录表达,但诱导能力有所差异,其中ABA诱导活性最强。A full-length cDNA encoding the C terminal binding protein gene OsCtBP-A, which is down-regulated dur- ing interaction between rice and Xanthomonas oryzae pv. oryzae （Xoo）, was cloned by 5＇-RACE. Bioinformaties was employed to elucidate the structure of this gene and its encoded protein. OsCtBP-A consists of 424 amino acids, with a 2-Hacid dh C domain in N terminal and NLS, PEST motif which are peculiar for plant CtBPs family in C terminal. Sequence alignment showed OsCtBP-A protein shares a 63% identity with homolognes of Arabiclopsis and Ipomoea nil. Furthermore, ABA and SA response elements were identified within the promoter of OsCtBP-A gene. Real-time quan- titative PCR （RTQ-PCR） showed that OsCtBP-A was induced by salicylic acid （SA）, ethane （ETH）, methyl jasmonate （MeJA） and abscisic acid （ABA） treatments with various expression levels respectively. OsCtBP-A tran- script increased drastically with ABA treatment.

关 键 词：水稻 C端结合蛋白 基因表达 信号分子 实时定量PCR 

分 类 号：Q786[生物学—分子生物学]