大麦黄矮病毒GPV株系基因组末端序列的克隆和分析  

Cloning and Analysis of the Terminal Sequence of Barley Yellow Dwarf Virus GPV

作　　者：张文蔚[1] 成卓敏[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国农业科技导报》2009年第1期102-107,共6页Journal of Agricultural Science and Technology

摘　　要：利用5′RACE、3′RACE和RT-PCR完成了大麦黄矮病毒GPV株系5′和3′末端序列的克隆和分析。分析结果显示,GPV株系5′末端长302 nt,包含起始密码子ATG和长100 nt的5′UTR。与马铃薯卷叶病毒属其他病毒比较,5′UTR的长度差异大且不保守。3′末端长328 nt,包含终止密码子TGA和长93 nt的3′UTR。比RPV3′UTR短74 nt,同源性为40%,但末端较为保守,与RPV3′UTR末端序列同源性达84.34%。The authentic 5＇ and 3＇ terminal sequences of genome of barley yellow dwarf virus （BYDV） GPV were obtained by 5＇ and 3＇ RACE and RT-PCR techniques. The analysis showed that 5＇ terminal sequence in the genome of GPV was 302 nt long, which included the start codon ATG and 5＇ un-translated region （UTR） of 100 nt. Compared with other virus of Polerovirus, the length of 5＇ UTR was variable and un-conservative. While 3＇ terminal sequence in the genome of GPV was 328 nt long, including the terminator codon TGA and 3＇ UTR of 93 nt. 3＇UTR was 74 nt shorter than that of RPV. The homology of 3＇UTR between GPV and RPV was 40%, but the terminal of 3＇ UTR was conservative. The homology was 84.34% between GPV and RPV.

关 键 词：大麦黄矮病毒GPV株系 5′RACE 3′RACE 5′UTR 3′UTR 

分 类 号：Q781[生物学—分子生物学]