基因工程ROSA小鼠主要脏器重量及脏器系数的测定  

The Measurement of Weight of Main Organs and Organ Coefficient in ROSA Genetically Engineered Mice

作　　者：田小芸[1] 恽时锋[1] 胡玉红[1] 周森妹[1] 

机构地区：[1]南京军区南京总医院比较医学科全军实验动物科普与伦理教育基地,南京210002

出　　处：《实验动物科学》2009年第1期1-3,共3页Laboratory Animal Science

摘　　要：目的测定基因工程B6;129-Gt(ROSA)26Sor小鼠主要脏器重量和脏器系数。方法实验选用5～6周雄性、6～7周雌性小鼠各15只,用Sartorius电子天平分别测定体重和10个主要脏器重量,计算脏器系数,并对雌雄脏器重量和脏器系数之间进行比较。结果雌雄小鼠脏器重量间比较,雄性鼠体重明显大于雌性的体重(P<0.01)、心、肾的重量间差异极显著(P<0.01);肝脏、肾上腺间比较差异显著(P<0.05)。雌雄间脏器系数比较,肝脏、肺脏、脑、肾、肾上腺间差异极显著(P<0.01),心、脾、胰脏间差异不显著(P>0.05)。结论转基因ROSA小鼠不同性别对脏器重量及脏器系数有一定的影响,同时该数据为其它相关研究提供参考。Objective To measure the organ weight and organ coefficient of Bs ;129-Gt （ROSA）26Sor mice. Methods 5 - 6 weeks male and 6 - 7 weeks female mice （ 15 in each group） were used in this experiment. The weight of various organs was measured using a Sartorius BP121S electronic balance. Results Between female and male mice, the body weight, the heart, kidney weight showed very significant difference （ P 〈 0.01 ） ; the liver, adrenal weight showed significant difference （ P 〈 0.05 ） ; the coefficient of liver, lung, kidney, brain, and adrenal showed very significant difference （ P 〈 0.01 ）, but no significant difference was seen in other organ coefficient. Conclusion The weight of some main organs and organ coefficient in different gender ROSA mice are significantly different; the above data could be useful in the biomedical researches.

关 键 词：基因工程小鼠 脏器重量 脏器系数 

分 类 号：Q95-33[生物学—动物学]