2型糖尿病伴随高脂血症大鼠模型的建立及二甲双胍的干预  

Rat Model Establishment of Type 2 Diabetes with Hyperlipidemia and the Intervention of Metformin

作　　者：饶子亮[1] 赵士海[1] 刘盛来[1] 刘科[1] 黄小琼[1] 钟志勇[1] 黎雄才[1] 

机构地区：[1]广东省医学实验动物中心,佛山528248

出　　处：《实验动物科学》2009年第1期15-18,共4页Laboratory Animal Science

摘　　要：目的研究5%蔗糖饮水和高脂饮食加链脲佐菌素诱导2型糖尿病伴随高脂血症大鼠模型的建立及二甲双胍的治疗作用。方法SD大鼠一次性腹腔注射30 mg/kg链脲佐菌素,给予高脂饮食和5%蔗糖水喂养68 d后进行糖耐量实验,采血检测空腹血糖、血脂、胰岛素、抗氧化水平。结果与对照组比较,模型大鼠空腹血糖、糖耐量试验2 h后血糖、糖化血红蛋白、血清胰岛素和胰岛素抵抗指数显著升高(P<0.05),胰岛素敏感指数显著降低(P<0.05),TG、TC和FFA含量明显升高(P<0.05),SOD活力明显降低(P<0.05),MDA含量显著增加(P<0.05);二甲双胍组胆固醇、游离脂肪酸、空腹血糖、糖耐量异常、糖化血红蛋白、血清胰岛素和胰岛素抵抗指数、MDA显著降低(P<0.05),胰岛素敏感指数和SOD活力显著提高(P<0.05)。结论5%蔗糖饮水能改善大鼠的厌食症状,加上链脲佐菌素的小剂量注射和高脂饮食,可成功复制2型糖尿病大鼠模型,伴随高脂血症和抗氧化损伤。二甲双胍具有降糖、降脂、抗氧化、改善胰岛素抵抗作用,可作为2型糖尿病伴随高脂血症大鼠模型研究的阳性治疗药物。Objective To establish a Rat model of Type 2 diabetes induced by streptozotocin supplemented with 5 % sucrose and high-fat diet, and to observe the therapeutic effects of metformin. Methods SD rats were injected peritoneally with streptozotocin （30 mg/kg） and 5 % sucrose and high-fat diet were given to the rats for 68 days, and were engaged in glucose tolerance test. Blood samples were taken to detect glucose, blood lipid, sarum insulin and anti- oxidation level. Results Compared with the control group, the glucose, tolerance test after 2h glucose, glycated hemoglobin, serum insulin and insulin resistance index in high-fat feeds group were significantly higher （P 〈 0.01 ）. The insulin sensitivity index was significantly lower （P 〈 0.01 ）. TG, TC and free fatty acids （FFA） were significantly higher （ P 〈 0.05）. The SOD activity decreased significantly （ P 〈 0.05）. MDA was significantly increased （ P 〈 0.01 ）. TC, FFA, glucose, glucose tolerance Anomaly, glycated hemoglobin, serum insulin and insulin resistance index, MDA In metformin-treated group were significantly lower （P 〈 0.05 ）. The insulin sensitivity index and SOD increased significantly （ P 〈 O. 05）. Conclusion 5 % sugar water can improve the symptoms of anorexia, and combined with the low-dose streptozotocin and high-fat diet haS successfully induced a type 2 diabetes rat model with hyperlipidemia. Metformin has a hypoglycemic, lipid-lowering, anti-oxidation effects, and can improve insulin resistance, So it can be used as a positive therapeutic drugs to treat type 2 diabetes rat model with hyperlipidemia.

关 键 词：2型糖尿病 高脂血症 大鼠模型 二甲双胍 链脲佐菌素 蔗糖水 

分 类 号：R543.1[医药卫生—心血管疾病]