大鼠心肌细胞的分离技术和电生理鉴定  

Isolation and Electrophysiological Characterization of Cardiomyocytes in Mouse Heart

作　　者：王腾[1] 唐其柱[1] 甘文云[1] 王蕾[1] 周恒[1] 田青[1] 

机构地区：[1]武汉大学人民医院心内科．武汉430060

出　　处：《科技导报》2009年第3期80-82,共3页Science & Technology Review

摘　　要：采用酶消化法分离单个心肌细胞,应用膜片钳全细胞记录技术记录钙电流,探索大鼠心肌细胞分离方法并进行电生理鉴定。结果显示,本方法分离所得心肌细胞横纹清晰,可获得90%～95%耐钙细胞,并具有正常电生理活性,易于形成高阻封接,可用于钙电流记录。本实验所采用的分离方法经济、简便、成功率高,并通过全细胞膜片钳记录证实该分离方法得到的细胞有正常的电生理特性。The methods of isolating cardiomyocytes in mouse heart and the electrophysiological characterization are studied in this paper. Enzyme digestion method was used to isolate single ventricular myocytes and whole cell patch clamp technique was used to record action potential and calcium current. The results show that 90%-95% pachyntic calcium tolerant ventricular myocytes can be obtained regularly, with a typical current of calcium channel recorded. The established method of isolation is simple, stable, effective and reliable. The electrophysiological recording of calcium current will provide a useful method for exploring the electrophysiological characteristics.

关 键 词：大鼠 心肌细胞 分离 膜片钳技术 

分 类 号：Q25-3[生物学—细胞生物学]