神经生长因子复合缓释剂的制备与性质研究  

Preparation and property of complicated controlled release drug of nerve growth factor

作　　者：于海龙[1] 彭江[1] 孙华燕[2] 徐风华[2] 张莉[1] 赵斌[1] 眭翔[1] 许文静[1] 卢世璧[1] 

机构地区：[1]解放军总医院全军骨科研究所,北京100853 [2]解放军总医院制剂室,北京100853

出　　处：《军医进修学院学报》2009年第1期73-75,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金(30571875);北京市自然科学基金重点项目资助(03G502)

摘　　要：目的:研制神经生长因子(nerve growth factor,NGF)复合缓释制剂,探讨其作为补充外源性NGF制剂的可行性。方法:采用NGF微球,与生物纤维蛋白胶混合形成NGF复合缓释制剂,分别进行体外、体内实验。体外实验包括药物释放曲线和释放药物的活性鉴定;体内实验为18只雄性W istar大鼠,根据修改缺损材料不同,随机分成3组(n=6):去细胞异体神经移植+NGF复合缓释制剂组(A组);化学去细胞异体神经移植组(B组):去细胞异体神经移植+纤维蛋白胶组(C组),术后2周测量神经轴突生长距离。结果:体外实验证明,NGF复合缓释制剂可持续释放NGF达60d以上,释出的NGF可促进鸡胚背根神经节神经纤维突起生长,具有较强的生物学活性。术后2周,测量再生神经生长距离分别为:A组(7.46±1.75)mm,B组(4.62±1.06)mm,C组为(4.36±1.20)mm,A组明显高于B、C组(P<0.05)。结论:制备的NGF复合缓释制剂,可长期释放NGF,并具有生物活性,可作为NGF良好的外源性补充制剂。Objective： To prepare the complicated controlled release drug vehicles of nerve growth factor （NGF）, and make experiments in vitro and in vivo in order to confirm whether it becomes drug vehicle of NGF. Methods： The microspheres of NGF were prepared drug microsphere technology and fixed with the fibrin gels to make the controlled release drug of NGF. In vitro, the drug release curve and bioactivity of complicated controlled release drug of NGF were made. In vivo, the 18 Wister rats were randomly divided into three groups： acellular nerve allograft ＋ the complicated controlled release NGF（ A group）, acellular nerve allograft （group B） and acellular nerve allograft ＋ fibrin glue （group C）. The animals were killed and the nerve regenera- tion lengths were measured in two weeks after operation. Results： The NGF was detected from the complicated controlled release drug of NGF at least 60 days and the bioactivity of released NGF stimulated the neuritis growth of chick dorsal root ganglion. In two weeks after operation, the axonal regeneration lengths were measured： A group was 7.46 ± 1.75mm, B group was 4.62 ±1.06mm and C group was 4.36 ± 1.20mm respectively. The lengths in A group were greater than those in B and C group （P 〈 0.05 ）. Conclusion： The complicated controlled release drug of NGF can release NGF for long time and protect the bioactivity of NGF. The method is good way to supply the exogenous NGF to improve the regeneration of nerve.

关 键 词：神经生长因子 纤维蛋白组织黏着剂 神经节 脊 

分 类 号：R622.3[医药卫生—整形外科]