模拟失重对肺微血管内皮细胞F-actin的影响  

Effects of simulated weightlessness on F-actin of pulmonary microvascular endothelial cells

作　　者：章烨[1] 袁明[2] 李婷[3] 张莹[3] 刘长庭[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853 [2]中国航天员科研训练中心,北京100193 [3]中国南方航空股份有限公司航空卫生管理部,广州510406

出　　处：《军医进修学院学报》2009年第1期91-92,共2页Academic Journal of Pla Postgraduate Medical School

基　　金：北京市科技计划课题(Z07050700690708);民用航天科研预先研究项目[科工技(2007)302]

摘　　要：目的:观察回转模拟失重对肺微血管内皮细胞纤维肌动蛋白(F-actin)的影响,探讨其在失重环境下的损伤机制。方法:组织块贴壁法原代培养肺微血管内皮细胞,采用回转器模拟失重效应。PMVEC回转培养24h、48h和72h,同时设1g同步对照静置培养。采用免疫荧光技术标记PMVEC内的F-actin,以激光共聚焦显微镜观察采集荧光图像。结果:与同步对照相比,不同时间回转的PMVEC微丝骨架均发生明显破坏,且这种破坏随着回转模拟失重时间的延长而加重。结论:模拟失重可破坏PMVEC内F-actin,导致PMVEC损伤。Objective： To investigate the effects of simulated microgravity induced by elinostat on filamentous actin （F-action） of pulmonary microvascular endothelial cells （PMVEC） and explore its mechanism. Methods： Clinostat was used to simulate the effects of microgravity. Pulmonary microvascular endothelial cells were cultured on clinostat for 24h, 48h and 72h, and then the F-actin of the cells were stained by Texas Red-X phalloidin. The images were collected by laser scanning confocal microscopy （LSCM）. Results： Clinostat induced the depolymerization of F-actin, which increased with the clinostat time. Conclusion ： Simulated microgravity can induce the destruction of cytoskeleton by increasing the depolymerization of F-actin and injuring PMVEC.

关 键 词：模拟失重 肺微血管内皮细胞 细胞骨架 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]