花粉管通道技术转化番木瓜的初步研究  

Preliminary Research of PRSV-CP Gene Transferred into Papaya by Pollen-tube Pathway

作　　者：蔡群芳[1,2] 魏军亚[3] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点实验室,中国海南海口571101 [2]海南医学院检验医学系,中国海南海口570102 [3]中国热带农业科学院热带作物品种资源研究所,中国海南儋州571737

出　　处：《生命科学研究》2009年第1期16-19,共4页Life Science Research

基　　金：国家自然科学基金资助项目(30760134);国家转基因植物研究与产业化开发专项资助项目(JY04-B-02);中央级事业单位基本科研业务费项目

摘　　要：以番木瓜"solo Ⅱ"号植株为受体材料,用花粉管通道法进行了番木瓜环斑病毒外壳蛋白基因(PRSV-CP)278bp片段的遗传转化.采用质粒DNA和农杆菌菌液两种导入液,分别处理花187和232朵,收获成熟番木瓜105和30个,座果率分别为56.15%和12.93%,随机选择每种载体的种子100粒播种,成株率分别为61%和60%.对T1幼苗(除含空载体外)全部进行PCR检测.检测结果为质粒DNA和农杆菌菌液两种导入液转化所得的幼苗阳性率分别为50.54%和51.22%.With Papaya line of solo Ⅱ as receptor, 278 bp fragment of Papaya ringspot virus coat protein （PRSV-CP） gene was transferred by pollen-tube pathway, which flowers were dealed with plasmid DNA （187 of flowers）and Agrobacterium bacilli （232 of flowers）. Thousands of seeds were gotten from 105 and 30 transferred fruits, which fruit rates were 56.15 percent and 12.93 percent respectively. With seed being sowed in greenhouses, each 100 seeds gotten from 8 kinds of transferred receptors were selected randomly, seedling rates of 61% and 60% were obtained separately. T1 plantlets were analyzed by PCR, which results showed that positive rates of transgenic plants were 50.54 percent and 51.22 percent, respectively.

关 键 词：番木瓜 花粉管通道法 PRSV—CP基因 PCR检测 

分 类 号：Q78[生物学—分子生物学]