不同日龄大白猪视黄醇结合蛋白基因的表达研究  

Research on Expression of Landrace RBP Gene on Different Days

作　　者：杨国伟[1] 张冬杰[1] 汪晓鸿[1] 王文涛[1] 刘娣 

机构地区：[1]黑龙江省农业科学院,中国黑龙江哈尔滨150086

出　　处：《生命科学研究》2009年第1期60-64,共5页Life Science Research

基　　金：黑龙江省杰出青年基金(JC-05-19);黑龙江省科技攻关项目(GB05B106)

摘　　要：采用半定量RT-PCR方法对大白猪的Retinol-Binding Proteins(RBP)基因在1、90、180、270、360日龄的心、肝、胃、脾、肾、肺、大肠、小肠、肌肉、子宫、卵巢共11个组织的表达情况进行了研究.结果表明RBP mRNA在肝脏、子宫和卵巢内持续表达,而且在肝脏中是持续高表达,子宫中RBP mRNA的表达在不同日龄间存在显著差异(P<0.05),且在90日龄时最低,脾脏不表达该基因,在胃里的表达时间较短,仅在1日龄时表达,心脏、肾脏、肺、大肠、小肠和肌肉组织中也表达RBPmRNA,但不同日龄不同组织的表达情况不一样,没有明显的规律性,但存在时间和空间的表达差异.Expression level of Retinol-Binding Proteins （RBP） gene in Landrace heart, liver, stomach, spleen, kidney, lung, large intestine, small intestine, muscle, uterus and ovary on 1, 90, 180, 270 d and 360 d were studied by half-quantity RT-PCR method. The result showed that RBP mRNA was continued to express in liver, uterus and ovary especially in liver. RBP mRNA was significant variance during different days in uterus （P〈0.05）, lowest on 90 d. It was not expressed in spleen, and expressed a short time in stomach on 1 d. Heart, kidney, lung, large intestine, small intestine and muscle also expressed RBP mRNA, they all had no obviously rules, but existed temporal and spacial difference.

关 键 词：大白猪 RBP RT.PCR 表达 

分 类 号：S813.1[农业科学—畜牧学]