高糖环境下脂联素对人肾小管上皮细胞转化生长因子-β1表达的影响  

Effect of adiponectin on the expression of transforming growth factorβ1 in human renal tubular epithelial cell cultured with high concentration of D-glucose

作　　者：刘玉芳[1] 李兴[1] 

机构地区：[1]山西医科大学第二医院

出　　处：《临床医药实践》2009年第2期104-106,共3页Proceeding of Clinical Medicine

摘　　要：目的：探讨脂联素（ADPN）对体外高糖培养的人肾小管上皮细胞转化生长因子-β1（TGF-β1）表达的影响，以揭示ADPN对高糖环境中的肾小管上皮细胞的可能保护作用机制。方法：人肾小管上皮细胞分别培养于不同的培养基中，在高糖环境中加入脂联素，分别培育48h后采用酶联免疫吸附法（ELISA）测定细胞上清液中TGF—β1的含量。结果：TGF—β1在高糖组表达较高，加入中等浓度（ADPN浓度为10．0，15．0，20．0ug／mL）TGF—β1的表达减少，和高糖组相比，两者的差异有统计学意义。结论：中等浓度ADPN可以抑制TGF—β1在人肾小管上皮细胞的表达。Objective：To investigate the effect of adiponectin （ADPN）on the expression of transforming growth factor-β1 （TGF-β1）in human renal tubular epithelial cell cultured with high concentration of D-glucose. Methods：Human renal tubular epithelial cells were cultured in media containing high concentration of D-glucose or adiponectin. The expression of TGF-β1 was measured by enzyme-linked immunosorbent assay（ELISA）at the 48 h. Results ：The expression of TGF-β1 was promoted in high glucose group. Compared with high glucose group ,the expression of TGF-β1 was suppressed in moderate concentration adiponectin（10.0,15.0,20.0 ug/mL）. Conclusion：Moderate concentration adiponectincan decrease the expression of TGF-β1.

关 键 词：脂联素:人肾小管上皮细胞 转化生长因子-1β 

分 类 号：R587.2[医药卫生—内分泌]