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作 者:张忠[1,2] 史卫峰[1] 叶恭银[2] 胡萃[2] 于爱莲[1]
机构地区:[1]泰山医学院,山东泰安271000 [2]浙江大学,浙江杭州310029
出 处:《中华卫生杀虫药械》2009年第1期28-31,共4页Chinese Journal of Hygienic Insecticides and Equipments
基 金:国家自然科学基金(30671825和30170626);泰山医学院博士科研启动基金
摘 要:目的研究利用冷冻蝇蛹繁殖丽蝇蛹集金小蜂的方法。方法观察丽蝇蛹集金小蜂对冷冻后3种蝇蛹的寄生率、出蜂率、出蜂数和雌蜂比。结果丽蝇蛹集金小蜂对冷冻后3种蝇蛹的寄生率显著降低,被寄生冷冻蝇蛹的出蜂率、出蜂数和丽蝇蛹集金蜂后代的雌蜂比显著下降,并随冷冻时间的延长,逐渐下降。结论-20℃冷冻比-70℃冷冻蝇蛹更适于丽蝇蛹集金小蜂的繁殖。Objective To study on the methods of breeding Nasonia vitripennis using the frozen fly pupae. Methods Three kinds of fly pupae, Boettcherisca peregrine,Musca domestica and Lucilia sericata were stored at - 20 ℃ and - 70 ℃ for different days. Based on laboratory, the parasitic and reproductive capability of Nasonia vitripennis was observed. Results The parasitic rate of frozen fly pupae, emergence rate and female rate of progeny of Nasonia vitripennis were decreased significantly. The decreased ratio had positive relationship with the frozen time. Conclusion Fly pupae frozen at -20 ℃ is better than the pupae frozen at -70℃ in breeding Nasonia vitripennis.
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