改良硝酸破机法检验尸体组织中的硅藻  

Improved Nitric Acid Digestion Method for Detecting Diatom in Autopsy Tissue

作　　者：杨颖峰 程冲 王政 林元益 林峰 马晓锋 陈煌[3] 

机构地区：[1]泉州市公安局,福建泉州362000 [2]泉州市公安局鲤城分局,福建泉州362000 [3]福建省公安厅,福建福州350000

出　　处：《法医学杂志》2009年第1期40-41,共2页Journal of Forensic Medicine

摘　　要：目的探讨改良硝酸破机法与传统硅藻检验方法的差异以及优点。方法无水乙醇充分固定尸体组织,取材80～100g,剪碎后使用硝酸破机,离心水洗去除有机成分后涂片镜检。结果硅藻纹理清晰,容易辨识,方便记数及种类甄别。结论改良硝酸破机法检验硅藻操作简单,检出率较高,标准化的实验过程可有效避免污染对实验结果的影响,提高实验结果的稳定性,避免实验过程中对人员和环境的危害,增加实验过程的安全性。Objective To investigate the advantages of nitric acid digestion method and its differences with the traditional method. Methods Ethanol was used to fully fix the testing sample. About 80-100 g of the testing samples were cut into pieces and digested with nitric acid. It was then centrifuged and washed to remove organic components. Smears were prepared and examined under the light microscope. Results The diatom had been identified with clear striations, counted conveniently and classified easily. Conclusion The improved nitric acid digestion method is not only simple with a higher successful rate of detection, but also can prevent interference from contamination. It can improve the stability of the experimental resuits, avoid harm to human and environment, and provide higher safety in the course of experiment.

关 键 词：法医病理学 溺水 硅藻 硝酸破机法 

分 类 号：DF795.4[医药卫生—法医学]