CT-1C-末端多肽对大鼠心肌缺血再灌注损伤(MI/R)心电图变化的影响  

Influence of cardiotroPhin-1 C-terminal PePtides on ECG in rats following myocardial ischemia rePerfusion injury

作　　者：陈淑芬[1] 姚震[1] 张晓芳[1] 

机构地区：[1]海南医学院附属医院,海口570102

出　　处：《实用心电学杂志》2009年第1期15-18,共4页Journal of Practical Electrocardiology

基　　金：海南省自然科学基金资助项目(编号:30214)

摘　　要：目的观察CT-1C-末端多肽(下简称"本药")不同干预方式对大鼠心肌缺血再灌注损伤心电图变化的影响。方法用结扎-松解SD大鼠冠状动脉左后降支的方法制作缺血再灌注损伤(MI/R)模型。27只SD大鼠随机分为4组:正常组(N组,n=5);疾病组(D组,n=6),心肌梗死(MI)30min后开始再灌注;MI/R后干预组(T组,n=8),MI30min后开始再灌注并腹腔注射本药100μg/kg;MI/R前干预组(O组,n=8),腹腔注射本药100μg/kg后进行MI/R实验。实验过程中,动态监测心电图,观察ST段、心率、P-R间期及心律失常的发生情况。结果①结扎前N组、D组、O组心率和P-R间期与N组无差别(F=0.6633,P>0.05),分别为:N组:396.43±29.34次/分、0.0480±0.0084s;D组:374.91±32.20次/分、0.0483±0.0075s;T组:390.57±21.20次/分、0.0488±0.0083s;O组:393.00±31.37次/分、0.0475±0.0089s。②结扎冠状动脉左后降支30min内,实际3组心率都明显减慢,P-R间期也明显延长,分别为:D组:254.61±44.60次/分、0.0733±0.0082s;T组:257.60±38.75次/分、0.0645±0.0101s;O组:285.65±48.92次/分、0.0638±0.0074s(与结扎前比较,q值分别为:6.8965、3.198;12.1344、4.573;4.5728、3.096;P<0.01)。再灌注2h内,D组和O组心率进一步减慢,P-R间期也进一步延长,前者为212.62±49.51次/分、0.0917±0.0313s,但与结扎后比较,差异不显著(q值为2.4073、2.755,P>0.05);后者为162.26±57.58次/分,0.1638±0.0717s,与结扎后有显著差异(q值为5.482、6.7452,P<0.01)。T组心率无明显变化,为246.16±30.51次/分,P-R间期有所延长,为0.0738±0.0106s但与结扎后比较,无显著差异(q值为1.044、2.6856,P>0.05)。③缺血30min内,仅D组有1只大鼠发生心律失常,发生率为:16.67%;再灌注后,三组大鼠心律失常的发生率均有增加,且各组间存在明显差别(X2=4.419,P<0.01),其中,T组发生率最低,为12.5%,O组发生率最高,为62.5%,D组为50%。结论①缺血及再灌注损伤均可导致SD大鼠心率减慢,P-R间期延长,并易于再灌注后发生心Objective Objective To study the change of ECG in rots following myocardial ischemia rePerfusion injury which were treated with CarditroPhin - 1 C - terminal PolyPePtides Pre - and Post - ischemia. Methods The ischemia rePerfusion heart model was established by ligating/loosening the left Posterior decending branch of coronary artery in SD rats. Matched Pairs of SD rats were treated with CT - 1 C - terminal PolyPePtides either before or after myocardial ischemia, and the ECG were monitered. Results The heat rate （HR） were decended and the P- R intermission were Prolonged in the rats of all grouPs after the coronaries were ligated, and the two Para were further decended and Prolonged in the ischemia rePerfusion grouP and Pre - ischemia grouP and were maintained unehange in the Post - isehemia group after the coronaries were loosened. The rate of arrhymia induced by ischemia rePerfusion injury were decreased in the Post - ischemia grouP but increased in Pre - ischemia group. Conclusion CarditrePhin - 1 C - terminal PolyPePtides Prevented the HR decending and P -R intermission Prolonging and decreased the rate of arrhymia induced by ischemia rePerfusion injury when it was administered at the same time of rePerfusiug.It accelerated the effects of ischemia rePerfusion injury on the HR and P - R intermission and increased the rate of arrhymia when it was administered choronically before ischemia.

关 键 词：CT-1C-末端多肽 缺血再灌注损伤 心电图 

分 类 号：R540.41[医药卫生—心血管疾病]