亚临界水萃取-酶抑制法快速检测蔬菜中的灭多威农药  

A Rapid Method Based on Subcritical Water Extraction and Acetylcholinesterase Inhibition Assay for the Determination of Methomyl Pesticide in Vegetables

作　　者：王琳玲[1] 王耀[1] 杨海[1] 陆晓华[1] 

机构地区：[1]华中科技大学环境科学研究所,湖北武汉430074

出　　处：《分析科学学报》2009年第1期90-92,共3页Journal of Analytical Science

基　　金：国家科技部十五科技攻关-食品重大专项(No.2001BA804A40);湖北省科技攻关计划项目(No.2005AA201C13)

摘　　要：本文研究了蔬菜中灭多威农药的亚临界水萃取-酶抑制法快速检测技术。研究表明,在萃取温度70℃,萃取时间5 min,提取液的pH为8.0,提取液体积为5 mL的条件下,灭多威有最高萃取效率。将本法分别应用于测定模拟样品和实际样品,结果表明亚临界水萃取的萃取效率和精密度都要高于国家标准方法中的手摇振荡萃取。A rapid method for the determination of methomyl pesticide in vegetables has been developed based on subcritieal water extraction （SWE） and acetylcholinesterase inhibition assay. The optimum extraction conditions were obtained by using 5mL of water （pH=8） as the extractant at 70℃ with 5 min static extraction. Compared with manual shaking extraction, subcritical water extraction has higher extraction efficiency and better precision in the determination of spiked and real samples.

关 键 词：灭多威 亚临界水萃取 乙酰胆碱酯酶抑制法 

分 类 号：O658.2[理学—分析化学]