四川鸽毛滴虫病调查和鸽毛滴虫体外保存方法  

Investigation of Trichomonas columbae Disease for pigeon in Sichuan Province and Research on in Vitro Preservative Method of T. columbae Body

作　　者：黄名英[1,2] 廖党金[3] 傅安静[2] 

机构地区：[1]西南民族大学动物科技学院,四川成都610066 [2]成都农业科技职业学院,四川成都611130 [3]四川省畜牧科学研究院,四川成都610066

出　　处：《西南农业学报》2009年第1期192-196,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家农业科技成果转化资金项目([2005]380)

摘　　要：采用随机抽样和镜检虫体法,调查了3个养鸽场和2个市场上的鸽毛滴虫病,在17000只肉鸽中,随机抽查1980只,鸽毛滴虫病感染率为75.12%,感染率的范围为34.44%-94.00%;信鸽50只,毛滴虫病的感染率为0%。为探索鸽毛滴虫的保存方法,就保存温度、保存液种类、保存液的pH值、保存液中的鸽毛滴虫密度、冷冻保护剂等对鸽毛滴虫的影响进行了测定,结果表明,保存溶液为生理盐水或pH7.2的PBS液、温度为28℃左右、溶液pH为7.2、保存液中的鸽毛滴虫密度为显微镜以10×40倍下每个视野50个-200个虫体为佳。The epidemic situations of Trichomonas columbae disease at 3 pigeon farms were investigated using the method of examining worm under microscope. A total number of 2030 pigeons randomLy selected from 17 000 were examined. The results showed the infective rate was 75.12 % and the range of infective rate were 34.44 % - 94.00 % at the 3 pigeon farms in Sichuan province. To obtain a in vitro preservative method, temperature, kind and pH of preservative liquid, density of T. columbae body in liquid and coolant affecting the parasite were measured . The results were that the in vitro preservative condition of T. columbae was the solution of 0.9 % sodium chloride or pH7.2 PBS, the temperature of about 28℃, the pH of 7.2 and the density of T. columbae body in liquid of 50 -200 bodies per field of microscope with 10 ×40 times.

关 键 词：鸽 鸽毛滴虫 保存方法 

分 类 号：S858.39[农业科学—临床兽医学]