Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production  

作　　者：夏金兰 孟浩 王润民 张成桂 熊晶 聂珍媛 邱冠周 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education, Central South University [2]School of Minerals Processing and Bioengineering, Central South University

出　　处：《Journal of Central South University》2009年第1期53-60,共8页中南大学学报（英文版）

基　　金：Projects(50621063, 50674101) supported by the National Natural Science Foundation of China

摘　　要：In order to solve the citrus peel resource waste problem protopectinase-overproducing strain CD-01 for pectin production and minimize the drawbacks of chemical extraction of pectin, a was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence （FJ184995） as that of Aspergillus niger （ATCC 64028）. It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L9（34） orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin is as follows： time 36 h, temperature 35 ℃, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus.In order to solve the citrus peel resource waste problem and minimize the drawbacks of chemical extraction of pectin, a protopectinase-overproducing strain CD-01 for pectin production was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence (FJ184995) as that of Aspergillus niger (ATCC 64028). It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L9(34) orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin is as follows: time 36 h, temperature 35 ℃, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus.

关 键 词：Aspergilllus niger pectin production protopectinase citrus peel fermentation optimization 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]