河南花背蟾蜍核型、Ag-NORs与性别关系  

Karyotype,Ag-NORs and Sex Determination of Bufo raddei from Henan

作　　者：董自梅[1] 孙小玲[1] 郭培军[1] 

机构地区：[1]濮阳职业技术学院,河南濮阳457000

出　　处：《河南师范大学学报（自然科学版）》2009年第1期164-166,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省高等学校青年骨干教师资助计划项目(2006-181)

摘　　要：利用骨髓细胞蒸汽固定法制备染色体标本,研究了分布于河南新乡的花背蟾蜍(Bufo raddei)的核型,Ag-NORs.结果表明:河南产花背蟾蜍体细胞染色体数为22条,核型公式为2n=22=20m+2sm,全部为中部或亚中部着丝点染色体,NF=44.Ag-NORs结果显示在性成熟的雌雄个体之间出现了稳定差异,提示河南花背蟾蜍可能为同形异配ZW型性别决定.The Chromosome is prepared from metaphase phase of marrow ceils by the steam-fixation method. Karyotype and Ag-NORs are studied on Bufo raddei which distributed in Xinxiang Henan. The diploid number of Bufo raddei is 2 n= 22 =20 m＋2sm,NF=44 and all of the chromosomes are metacentric or submetacentric. Stable diversity of female and male is found in Ag-NORs ,showing the sex determination about Bufo raddei is ZW-type.

关 键 词：花背蟾蜍 核型 Ag—NORs 性别决定 

分 类 号：Q953[生物学—动物学]