MCS^+增强型血细胞分离机采集外周血造血干细胞探讨  

Preliminary study on peripheral blood stem cells collected by blood cell separator MCS^+

作　　者：詹廷西[1] 张国珍[1] 谭兵[1] 

机构地区：[1]重庆医科大学附属第一医院输血科,400016

出　　处：《重庆医学》2009年第4期464-465,467,共3页Chongqing medicine

摘　　要：目的初步探讨增强型血细胞分离机(MCS+)在采集外周血造血干细胞(peripheral blood stem cells,PBSC)中的应用,对影响采集的相关因素进行分析。方法总结本院2004~2007年26例自/异体共48次PBSC采集,分析采集成功率、采集物的各项指标、参数设定及各种影响因素。结果26例患者其中3例(11.5%)行肘静脉穿刺,23例(88.5%)股静脉插管。每次平均采集时间(342±43)min,处理全血量(10 128±1 877)mL,平均循环数32±8,终产品(113±25)mL,单个核细胞数(MNC)(148.88±46.47)×109/L,MNC采集率(93.2±2.4)%,CD34+细胞数(7.91±3.48)×106/kg。26例患者中4例采集1次,22例连续采集2次,共48次采集,全部病例均获造血功能重建,无移植相关死亡。结论采用MCS+采集PBSC,应根据不同患/供者的实际情况,合理设置各种采集参数、选择适当穿刺部位,经1~2次采集,均能收集到足够高质量的PBSC并安全用于移植,满足临床治疗要求。Objective To study the using of MCS＋ blood cell collection machine to harvest peripheral blood stem cells and to analyse the correlation factors affecting gathering. Methods Totally 26 patients or donors were received PBSCT treatment from 2004 to 2007 and48 harvests. The incidence of successful collection, each gathering target, parameter setting and influential factors were analyzed. Results Among 26 patients, 3 patients（11.5% ） were through elbow vein while the other 23 patients（88. 5%） were through thigh vein. The average time was （ 342 ± 43） min, blood volume disposed was （ 10128 ±1877 ） mL, circulation frequency were （32±8） ,the volume of production was （113 ± 25） mL, mononuclear cell was （148.88±46.47））〈 109/L, collection efficiency of MNC was （93.2±2.4）% and CD34＋ cells were （7.91±3.48） × 10^6/kg. Among 26 gatients,4 patients were collected once and other 22 patients received twice collection. All the patients procured reconstructed haematogenesis function,and there was no transplantation related death. Conclusion According to situation of different patient/donor,establishing each kind of gathering parameter in reason,chosing suitable puncture spot and gathering once or twice, can collect high quality PBSC by using MCS＋ blood cell and use in transplanting safely to meet clinical requirement.

关 键 词：MCS+ 外周血造血干细胞 血细胞分离机 采集 

分 类 号：R457[医药卫生—治疗学]