两步培养法提高蛋白核小球藻的油脂含量  

Improvement of Lipid Content of Chlorella pyrenoidosa by Two-Step Cultivation

作　　者：黄冠华[1] 陈峰[1] 魏东[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2008年第12期97-101,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金-广东省自然科学基金联合基金重点项目(U0633009);国家科技部"十一五"科技支撑计划项目(2006BAD27B03);广东省科技攻关项目(20052050166);广州市科技计划项目(2005Z3-E0331)

摘　　要：基于Chlorella pyrenoidosa的生长规律和在缺氮条件下培养可提高其细胞内的油脂积累的特性,进行了两步培养法提高小球藻油脂产量的研究.结果显示:小球藻高密度生长的最适葡萄糖和硝酸钾质量浓度分别为20 g/L和1.50 g/L;单步培养法中氮源KNO3初始质量浓度为1.50 g/L时的小球藻生物量为(7.55±0.23)g/L,两步培养法中的小球藻生物量提高至(9.23±0.11)g/L;与单步培养法相比,两步培养法中小球藻细胞的油脂含量和总脂肪酸含量分别提高了5.8%和5.2%,藻的热值由(23.58±0.02)kJ/g提高到(25.52±0.03)kJ/g.这说明,两步培养法不仅可保证藻细胞的高密度生长,而且能有效增加藻细胞的油脂含量.Based on the growth rules of Chlorella pyrenoidosa （ C. pyrenoidosa） and the characteristics of intracellular lipid accumulation in nitrogen-deficient condition, the improvement of lipid content of C. pyrenoidosa using a two-step cultivation method was investigated. The results show that （ 1 ） the optimal mass concentrations of glucose and KNO3 for the high-density cultivation of C. pyrenoidosa are respectively 20 and 1. 50 g/L; （2） with 1.50 g/L KNO3 as the nitrogen source, the biomass of C. pyrenoidosa increases from （7.55 ± 0. 23 ） g/L in the single-step cultivation to （9. 23 ±0. 11 ） g/L in the two-step cultivation; （3） the contents of intracellular lipid and total fatty acids in the two-step cultivation .respectively increase by 5.8% and 5.2% ; and （4） the calorific values improves from （23.58 ±0. 02） kJ/g to （25.52±0.03） kJ/g. It is thus concluded that the two-step cultivation not only results in a high-densitv cultivation of C. pyrenoidosa but also effectively increases the cellular linid content.

关 键 词：小球藻 油脂 氮缺失 热值 两步培养法 

分 类 号：Q175[生物学—水生生物学] Q949.21[生物学—普通生物学]