11种野生苹果种质资源RAPD标记研究  

A PRELIMINARY STUDY ON RAPD MARKERS OF WILD SPECIES IN MALUS MILL.

作　　者：周志钦[1] 李育农[1] 王力超[1] 

机构地区：[1]西南农业大学园艺系

出　　处：《西南农业大学学报（自然科学版）》1998年第1期34-37,共4页Journal of Southwest Agricultural University

摘　　要：利用Ｃ．Ｇ．Ａｌｐｈａ等的ＤＮＡ微量提取法，提取了山荆子ＭａｌｕｓｂａｃｃａｔａＢｏｒｋｈ．等１１个苹果属植物野生种类的ＤＮＡ，并用５对引物对所研究材料的ＲＡＰＤ标记进行了研究。探讨了叶片中富含多酚类化合物的苹果植物叶片ＤＮＡ的提取及纯化方法，并初步建立了野生苹果ＲＡＰＤ标记的ＰＣＲ反应条件。The DNA of eleven wild species from Malus Mill. were extracted by using a new method developed by Christopher G.Alpha etc. Five pairs of primers of group P from Operon Company were used to establish the RAPD markers of the species. As a result, We got the RAPD markers of five out of 11 species, and established the reaction parameters for PCR of Malus wild species. The problems were discussed of isolating DNA from plant tissues rich in polyphenolics, like the leaves of Malus species.

关 键 词：苹果 野生种 种质资源 RAPD标记 

分 类 号：S661.102.4[农业科学—果树学]