一株纤维素降解菌的分离、鉴定及降解特性初步研究  

Screening and Characterization of Cellulase-Producing Aspergillus Fumigatus NX1-1

作　　者：夏金兰[1] 张瑞永[1] 张倩[1] 彭娟花[1] 张成桂[1] 熊晶[1] 武顺[1] 金德才[1] 

机构地区：[1]中南大学资源加工与生物工程学院生物冶金教育部重点实验室,中国长沙410083

出　　处：《湖南师范大学自然科学学报》2008年第4期86-90,共5页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(50621063;50674101);国家创新研究群体科学基金资助项目(50321402)

摘　　要：从长沙宁乡灰汤温泉附近的水样中分离获得1株具有较强纤维素降解能力的菌株,命名为NX1-1,该菌株最适生长温度为40℃,最适生长pH5.0-8.0.菌株形态学以及ITS rDNA和28S rDNA D1/D2序列分析的鉴定结果表明,该菌株属于Aspergillus fumigatus这个种.对该菌株产酶特性进行了初步研究,最适产酶条件为：氮源（NH4）2SO4,接种量10%,40℃,初始pH6.0,培养60-96 h.A cellulose-producing strain named NXI-1 was isolated from environmental samples collected at hot springs of Ningxiang County, Hunan Province, China. The temperature for growth was 40 ℃ and pH values ranging from 5.0 to 8.0. The cell shape suggested that strain NXI-1 was likely a strain of Aspergillus fumigatus. Furthermore, A phylogenetic analysis based on ITS rDNA and 28S rDNA D1/D2 sequences showed that the strain NXI-1 should be classified into the species Aspergillus fumigatus. The most suitable conditions for the cellulose production were ： inorganic nitrogen source （ NH4 ） 2SO4, 10% of inoculation, 40℃, initial pH at 6.0, culture for 60 - 96 h.

关 键 词：分离鉴定 系统发育分析 烟曲霉 产酶条件 

分 类 号：Q939[生物学—微生物学]