青藤碱对类风湿关节炎患者外周血树突状细胞TLR2和TLR4表达的影响  

Effect of sinomenine on expression of TLR2 and TLR4 of dendritic cells in rheumatoid arthritis

作　　者：余克强[1] 罗仁[1] 

机构地区：[1]南方医科大学中医药学院,广州510515

出　　处：《中华中医药杂志》2009年第3期316-319,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助(No.30400614);广东省中医药管理局课题资助(No.2007118)

摘　　要：目的:观察中药单体青藤碱对类风湿关节炎患者外周血树突状细胞toll样受体(toll like receptor,TLR)2和TLR4表达的影响。方法:分离10例类风湿关节炎患者的单核细胞,采用细胞因子刺激分化为树突状细胞。分别采用青藤碱高(5mM)、中(2mM)、低(1mM)剂量和空白对照干预。实时荧光定量PCR检测各组树突状细胞TLR2和TLR4 mRNA的表达,Western-blot检测树突状细胞TLR2和TLR4蛋白的表达。结果:与对照组相比,经青藤碱高(P<0.01)、中剂量(P<0.01)干预后的树突状细胞TLR2和TLR4 mRNA表达明显降低,青藤碱干预后的树突状细胞(DC)中TLR2、TLR4表达均有所有降低。结论:青藤碱可能通过抑制树突状细胞TLR介导的炎症信号转导,而产生治疗类风湿关节炎的作用。Objective： To investigate the effect of sinomenine on expression of toll like receptor （TLR） 2 and TLR4 of dendritic cells in patients with rheumatoid arthritis in vitro. Methods： The peripheral blood mononuclear cells were isolated from 10 rheumatoid arthritis patients and differentiated into dendritic cells by stimulating with cytokines. Dendritic cells were exposed to high （5mM）, middle （2mM）, low （1mM） concentration sinomenine and control medium. TLR2 and TLR4 mRNA were detected by real time PCR. Western blot was used to measure the expression of TLR2 and TLR4. Results： In contrast to control, significant lower expression of TLR2, TLR4 mRNA and protein were found in dendritic cells treated with sinomenine, especially in high and middle concentration. Conclusion： The therapeutical effect of sinomenine on rheumatoid arthritis is probably by inhibiting dendritic cells expressing TLR, which can transduce the inflammatory signal.

关 键 词：类风湿关节炎 青藤碱 树突状细胞 TOLL样受体 

分 类 号：R259[医药卫生—中西医结合]