糖肾汤对氧化低密度脂蛋白诱导的系膜细胞血凝素样氧化低密度脂蛋白受体-1表达及细胞外基质分泌的影响  

Effects of Tangshentang on the expression of LOX-1 and secretion of extracellular matrix induced by ox-LDL in rat masengial cells

作　　者：杜月光[1] 柴可夫[1] 赵天喜[1] 

机构地区：[1]浙江中医药大学基础医学院,杭州310053

出　　处：《中华中医药杂志》2009年第3期320-323,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金项目(No.M303727)

摘　　要：目的:观察糖肾汤对氧化低密度脂蛋白(ox-LDL)诱导的系膜细胞血凝素样氧化低密度脂蛋白受体1(LOX-1)表达及转化生长因子-β1(TGF-β1)和细胞外基质分泌的影响,探讨其防治糖尿病肾病的机制。方法:培养大鼠系膜细胞,分为空白组、ox-LDL组、糖肾汤高、中、低剂量组和罗格列酮组,培养24h后,RT-PCR检测系膜细胞mRNA表达,ELISA技术检测细胞上清液中TGF-β1、四型胶原(ColⅣ)和纤黏连蛋白(FN)含量。结果:糖肾汤、罗格列酮含药血清均能抑制ox-LDL诱导的TGF-β1、LOX-1 mRNA表达上调,及TGF-β1、FN、ColⅣ的分泌,其中糖肾汤含药血清呈剂量依赖性作用,中浓度作用最强。结论:糖肾汤可能通过降低ox-LDL受体LOX-1的表达以减少ox-LDL摄入及由此引起的细胞因子分泌和细胞外基质产生,从而起到防治糖尿病肾病的作用。Objective： To investigate the effect of Tangshentang on the expression of LOX-1 and secretion of TGF- β1 .FN,CollV in cultured rat masengial cells （RMCs） induced by ox-LDL and to explore its molecular mechanism of preventing and treating diabetic nephropathy. Methods： The cultured RMCs were divided into control group, ox-LDL group, Tangshentang group （respectively high, medium and low group）and rosiglitazone group. The mRNA expression was tested by RT-PCR. Synthesis of TGF- β1.FN,ColIV in cultured RMCs were determined by ELISA methods. Results： Tangshentang drug serum significantly attenuated up-regulation of TGF- β1,LOX-1 mRNA expression and synthesis of TGF- β1,FN,ColⅣ in a dosedependent manner, with the peak at middle concentration, in RMCs stimulated by ox-LDL（50μg/ml）. Conclusion： Tangshentang drug serum may protect kidney from injury by ox-LDL via the decreased expression of LOX-1 to reduce the uptake of ox-LDL and subsequently inhibiting TGF- β1 secretion ,as well as the deposition of FN,ColⅣ.

关 键 词：糖肾汤 氧化低密度脂蛋白 血凝素样氧化低密度脂蛋白受体1 肾系膜细胞 转化生长因子-β1 细胞外基质 

分 类 号：R285.5[医药卫生—中药学]