荷叶生物碱部位大鼠血中移行成分的初步研究  

Study on adscription of plasma effective constituents of rat after administrated with alkaloid fraction of Nelumbo nucifera

作　　者：吴昊[1] 刘斌[1] 王伟[1] 石任兵[1] 王玉霞[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中华中医药杂志》2009年第3期324-326,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"十一五"科技支撑计划项目(No.2006BAI08B03-04);国家自然科学基金课题(No.90409011);北京市自然科学基金课题(No.7061002)

摘　　要：目的:初步研究荷叶生物碱部位大鼠给药后血中的移行成分。方法:建立荷叶生物碱部位大鼠给药后血中移行成分HPLC分析方法;比较荷叶生物碱部位,给药后所得血浆样品及空白血浆样品色谱图,初步指认出荷叶生物碱部位大鼠血浆中的移行成分。结果:在实验条件下,检测到16个血中移行成分,其中3个为原型成分直接入血,13个为代谢产物。紫外图谱显示的各峰最大吸收波长与原型物质最大吸收波长比较结果提示,7个代谢产物最大吸收波长与原型相同,表明其基本骨架未发生变化,6个代谢产物最大吸收波长变化较大,表明结构变化较明显。结论:通过对荷叶生物碱部位血中移行成分进行分析,可初步推测其代谢产物的结构,并将有助于对其体内吸收、分布、代谢、排谢及作用机制的研究。Objective： To study the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera. Methods： Based on the established HPLC analytical method of plasma effective constituents, analysis and comparison were carried out among HPLC profiles of plasma samples obtained after oral administration of alkaloid fraction of Nelumbo nucifera, blank plasma, alkaloid fraction of Nelumbo nucifera. Results： Sixteen compounds were detected under the method, three of them were architypes of compound contained in alkaloid fraction of Nelumbo nucifera, and the others were metabolites. Through the comparison of the UV spectra, seven metabolites remain essential structure because the max wavelength was same to the architypes＇, the other six metabolites＇ structure changed greatly. Conclusion： Through the study of the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera, the structure of metabolites can be conjectured ,which may be helpful to study the ADME and mechanism of action.

关 键 词：荷叶 生物碱部位 移行成分 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]