保元Ⅱ号对糖尿病大鼠肾脏中Smad蛋白表达的影响  

Effect of BaoYuan Ⅱ on expression of Smad in Kidney of diabetic rat

作　　者：李青[1] 张鹤[1] 丁振华[2] 闫慧明[1] 

机构地区：[1]石家庄市中医院肾内科,石家庄050051 [2]石家庄市第二医院急诊室,石家庄050051

出　　处：《中华中医药杂志》2009年第3期370-372,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河北省科技厅课题(No.07276101D-24)

摘　　要：目的:观察Smad在糖尿病大鼠肾脏中的表达及保元Ⅱ号对其表达的影响。方法:用链脲佐菌素腹腔注射制备糖尿病大鼠模型,随机分为空白对照组(N),模型组(DN),保元Ⅱ号治疗组(Z)和贝那普利治疗组(B),并用相应的药物干预,给药8周后处死大鼠,检测各组血浆白蛋白、血糖、尿蛋白定量、尿β2微球蛋白含量,并且用RT-PCR检测TGF-β1和Smad2,Smad3,Smad7在肾组织的表达水平。结果:与DN组相比,DN-Z组血浆白蛋白明显上升(P<0.01),血糖和尿蛋白定量、尿β2微球蛋白水平均有下降(P<0.01);TGF-β1、Smad2、Smad3的mRNA在模型大鼠肾组织中表达明显下调,Smad7的mRNA明显下调。干预效果与贝那普利相近。结论:保元Ⅱ号对糖尿病大鼠肾脏有保护作用,其机制可能与调节肾脏中TGF-β1和Smad2,Smad3,Smad7表达水平有关。Objective： To observe the expression of Smad in diabetic rat＇s kidney and the effect of BaoYuan Ⅱ to it. Methods： SD rats were randomly divided into four groups： control group, model group, Benazepril-treatment group and BaoYuan Ⅱ-treatment group. The latter three groups were induced to be diabetic nephropathy by intraperitoneal injection with streptozotocin and then the drugs were treated respectively. After eight weeks, the plasma albumin, blood glucose, urinary protein, urinary β 2-M were determined and expression of mRNA of TGF- β1, Smad2, Smad3, Smad7 was detected by RT-PCR. Results： Compared with diabetic rats group, the level of plasma albumin was increased and the level of blood glucose, urinary protein, urinary β 2-M was depressed in BaoYuan H-treatment group. The expression of TGF- β1 and Smad2, Smad3 were down- regulated and Smad7 was up-regulated in model group. The effects of BaoYuan II were similar to Benazepril. Conclusion： BaoYuan II had protected effect to diabetic nephropathy rats. It acted by regulating TGF- β 1 and Smad2, Smad3, Smad7 possibly.

关 键 词：糖尿病肾病 中药 TGF—β1 SMAD2 SMAD3 SMAD7 

分 类 号：R285.5[医药卫生—中药学]