雷公藤固态生物转化产物的初步药理研究  

Preliminary pharmacological studies on the bioconversion product of Tripterygium wilfordii

作　　者：马伟光[1] 毕云[1] 黄之镨[1] 张超[1] 叶建州[1] 赵云丽 钱如贵 张炜 张小贵 

机构地区：[1]云南中医学院,昆明650200 [2]云南药物研究所,昆明650111

出　　处：《中华中医药杂志》2009年第3期373-376,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助(No.30660223);云南省财政厅资助;云南省政府改善办学条件专项资金资助

摘　　要：目的:对3种雷公藤生药整体固态生物转化后的分段提取物进行抗炎、免疫药理作用比较,初步判断生物转化产物对药效的影响。方法:二甲苯致小鼠耳廓肿胀、免疫器官重量测定、2,4-二硝基氟苯(DNFB)致小鼠迟发性超敏反应实验。结果:转化后的甲醇总提物抗炎作用比未转化者强,尤其是3#菌种转化总提物的作用最强;TW2和TW3的醋酸乙酯部分对小鼠胸腺系数呈增加趋势;TW3的石油醚和氯仿部分对免疫器官、醋酸乙酯部分对细胞免疫的抑制作用增强。结论:转化后样品与未转化的对照品在抗炎、免疫作用上发生了一定变化,提示转化产物中的活性物质基础可能有所改变。To research the antiinflammatory and immunological pharmacology effect of bioconversion product of crude Tripterygium wilfordii （ TW ） . Methods ： The mice＇ experiments of swelling of auricle induced by dimethyl benzene, weight determination of immune organs, delayed hypersensititivity reaction induced by DNFB were selected.Results： The effect of total MeOH extractive of bioconversion product was better than that of non-transformed product, especially the TW3（MeOH） extractive. The TW2 （ EtOAc ） and TW3 （ EtOAc ） increased thoracic gland coefficient of mouse. The TW3（DAB-6）,TW3 （ CHCl3 ） and TW3 （ EtOAc ） increased the inhibitory action on peripheral immune organs and cell immunity of mouse. Conclusion：The antiinflammatory and immunological pharmacology effect of TW had changed after biotransformation, which indicated that the active compound basis of transformed products may be different.

关 键 词：雷公藤 生物转化 药效研究 

分 类 号：R285.5[医药卫生—中药学] R978.11[医药卫生—中医学]