痹祺胶囊对类风湿关节炎外周血单核细胞人类软骨糖蛋白39的影响  

Effect of Biqi capsule on human cartilage glycoprotein-39 in peripheral blood monouclear cells in rheumatoid arthritis

作　　者：刘维[1] 刘晓亚[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《中华中医药杂志》2009年第3期391-392,共2页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：天津市应用基础研究计划面上项目(No.06YFJMJC09600)

摘　　要：目的:通过观察痹祺胶囊对人类软骨糖蛋白-39(HCgp-39)mRNA在外周血单核细胞(PBMC)的表达水平,研究痹祺胶囊的可能作用机制。方法:服用痹祺胶囊的类风湿关节炎(RA)患者39例及健康查体者10名,取新鲜抗凝静脉血,分离单个核细胞,逆转录半定量PCR检测HCgp-39 mRNA在PBMC的表达水平。结果:RA患者HCgp-39 mRNA明显表达,其相对表达量为(0.7920±0.5240),与健康对照组比较有显著性差异(0.3104±0.2519,P=0.024);经治疗后HCgp-39 mRNA的表达水平有显著下降,比较有显著性差异(0.4755±0.2431,P=0.038);结论:HCgp-39 mRNA在RA患者PBMC表达明显高于健康正常人,提示单核细胞HCgp-39 mRNA与RA活动性的相关性,治疗后其表达水平有了一定的下降,提示痹祺胶囊的免疫调节作用。Objective： To observe the effect of Biqi capsule on human cartilage glycoprotein-39 in peripheral blood monouclear cells, research the possible mechanism of Biqi capsule.Methods： 39 patients with RA and 10 healthy people were selected.Expression levels of human cartilage glycoprotein-39 （HCgp-39） mRNA in peripheral blood monouclear cells （PBMC） were detected by Rt-PCR method.Results： Obvious expression of HCgp-39 mRNA was found in patients with RA ,the relative expression quantity was（0.7920±0.5240）, which has a significant difference （0.3104±0.2519, P=0.024）compared with the healthy controls; expression levels of the HCgp-39 mRNA after treatment were significantly decreased, there were statistically significant differences（0.4755±0.2431, P=0.038） between pre and post treatment.Conclusions： The HCgp-39 mRNA in PBMC of patients with RA were obviously higher than that of healthy ones. These data supported a correlation between the activity of RA and the HCgp-39 mRNA in PBMC; there was a certain fall in the expression levels after treatment, which hinted the immunological regulation of Biqi capsule.

关 键 词：痹祺胶囊 类风湿关节炎 单核细胞 人类软骨糖蛋白-39 

分 类 号：R259[医药卫生—中西医结合]