前列通瘀联合强的松对免疫性不育雄鼠睾丸超微结构的影响  

Effect of Qianlietongyu plus prednisone acetate on ultrastructure of testis in immune infertility mice

作　　者：王璟琦[1] 王东文[1] 徐计秀[1] 张利[1] 王建波 

机构地区：[1]山西医科大学第一临床医学院泌尿外科,太原030001 [2]榆社县人民医院外科

出　　处：《山西医科大学学报》2009年第2期110-113,共4页Journal of Shanxi Medical University

摘　　要：目的研究中药前列通瘀联合强的松对免疫性不育雄性小鼠的睾丸超微结构的影响。方法用同种小鼠精子及福氏佐剂免疫昆明种雄性小鼠获得免疫性不育动物模型。模型小鼠分为四组:联合组、前列通瘀组、强的松组和生理盐水组。四组分别给予前列通瘀加强的松、前列通瘀、强的松、生理盐水,灌胃1个月,同时设立正常对照组给予生理盐水灌胃1个月;ELISA法检测血清抗精子抗体(AsAb),透射电镜观察各组睾丸的超微结构。结果人工免疫后的雄鼠血清AsAb均为阳性,正常对照组均为阴性。前列通瘀联合强的松组血清AsAb的A值低于前列通瘀组、强的松组与生理盐水组(P<0.05),睾丸超微结构则优于其他三组(P<0.05),且与正常对照组比较无统计学差异(P>0.05)。结论AsAb可通过攻击睾丸生精微环境影响雄性小鼠的生育力。前列通瘀联合强的松可明显改善免疫性不育雄性小鼠生精微环境。Objective To investigate the effect of Chinese herbal formula-Qianlietongyu plus prednisone acetate on uhrastructure of testis of immune infertility mice. Methods The mice were injected with mouse sperm and Fruend adjuvant for establishing the immune infertility mouse model. The model mice were divided into 4 groups at random, and were administered with Qianlietongyu, prednisone acetate, Qianlietongyu plus prednisone acetate ,0.9% sodium chloride, respectively. At the same time, the normal control mice were administered with 0.9% sodium chloride. The ultrastructure of testis was observed and AsAb in serum was determined by ELISA. Results The AsAb in serum showed positive in all model mice, but negative in normal control mice. The A value of AsAb in mice treated with Qianlietongyu plus prednisone acetate was lower than that of the other model mice （ P 〈 0.05 ）, and the ultrastructure of testis was superior to that of the other model mice, but there was no significant difference from the normal controls （ P 〉 0.05 ）. Conclusion AsAb can result in male mouse infertility by attacking the spermatogenesis microenviroment. Qianlietongyu plus prednisone acetate can improve ultrastructure of testis.

关 键 词：免疫性不育 生精微环境 小鼠 

分 类 号：R711.6[医药卫生—妇产科学]