再生障碍性贫血小鼠Foxp3的表达及意义  

Expression of Foxp3 in aplastic anemia mice and its significance

作　　者：孟丽娟[1] 张秀莲[1] 张伟华[1] 范星火[1] 陈玮[1] 侯素敏[1] 陶洁[1] 刘晨[1] 

机构地区：[1]山西医科大学第一临床医学院血液科,太原030001

出　　处：《山西医科大学学报》2009年第1期35-38,96,共5页Journal of Shanxi Medical University

摘　　要：目的探讨再生障碍性贫血(简称再障)小鼠外周血单个核细胞调节T细胞转录因子Foxp3的表达及其意义。方法①采用免疫介导(γ射线照射和淋巴细胞输入)的方法建立再障小鼠模型。②RT-PCR方法检测再障小鼠外周血单个核细胞Foxp3 mRNA的表达。③免疫组织化学方法检测再障小鼠脾脏组织Foxp3蛋白水平的表达。结果①再障小鼠外周血单个核细胞Foxp3 mRNA的表达较正常小鼠降低(18.90±2.45vs57.27±5.71,P<0.05)。②Foxp3蛋白表达水平在再障小鼠脾脏中较正常小鼠低(19.2±10.0vs51.4±38.8,P<0.05)。结论Foxp3在再障小鼠外周血中及脾脏组织中表达明显减少,提示Foxp3的表达降低可能参与再生障碍性贫血的免疫发病机制。Objective To explore the expression of transcription factor Foxp3 of regulatory T-cell in peripheral blood mononuclear cells in aplastic anemia mice. Methods （1）Immune-mediated method（γ-ray radiation and lymphocytes injection）was used to establish the model of aplastic anemia in BALB/c mice. （2）The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was measured by RT-PCR. （3）The expression of Foxp3 protein in spleen of aplastic anemia mice was detected by immunohistochemical method. Results （1）The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was lower than that in normal mice （ 18.90 ± 2.45 vs 57.27 ±5.71, P 〈 0.05 ）. （2）The expression of Foxp3 protein in the spleen of aplastic anemia mice was lower than that in normal mice（ 19.2±10.0 vs 51.4 ± 38.8 ,P 〈0.05）. Conclusion The results suggest that the expression of Foxp3 may be involved in the immune pathogenesis of aplastic anemia.

关 键 词：再生障碍性贫血 免疫介导 FOXP3 RT-PCR 免疫组化 

分 类 号：R556.5[医药卫生—血液循环系统疾病]