铬天青S-氟罗沙星分光光度法测定粉针剂中氟罗沙星的含量  

Chrome azurol S-fleroxacin spectrophotometry for determining the contents of fleroxacin in powder for injection

作　　者：全红[1] 白小红[1] 王晓芳[1] 

机构地区：[1]山西医科大学药学院药物分析教研室,太原030001

出　　处：《山西医科大学学报》2009年第1期60-63,共4页Journal of Shanxi Medical University

基　　金：山西省自然科学基金资助项目(2007011086)

摘　　要：目的铬天青S-氟罗沙星分光光度法测定粉针剂中氟罗沙星的含量。方法在一定条件下,氟罗沙星与铬天青S反应生成在518nm处有最大吸收的n-π型1∶1电荷转移化合物或n-σ*型1∶1卤键化合物,利用铬天青S-氟罗沙星分光光度法测定氟罗沙星含量。结果氟罗沙星的线性范围为0.24-19.20mg/L(r=0.9997),检测限为0.018mg/L,精密度RSD=1.0%,方法回收率为(101±6.5)%。结论天青S-氟罗沙星分光光度法灵敏、简便、快速,可用于粉针剂中氟罗沙星的含量测定。Objective To determine the content of fleroxacin in powder for injection with chrome azurol S-fleroxacin spectrophotometry. Methods Under certain conditions, a molar ratio 1：1 n-π charge transfer or n-σ halogen bonding compound at 518 nm was formed between fleroxacin and chrome azurol S. The content of fleroxacin was determined by chrome azurol S-fleroxacin spectrophotometry. Results Linear range, detection limit, precision （RSI）） and recovery for fleroxacin were 0.24 - 19.20 mg/L（ r = 0. 999 7 ） ,0.018 mg/L,1.0 and （101 ±6.5 ）%, respectively. Conclusion Chrome azurol S-fleroxacin spectrophotometry is a sensitive, simple and fast method, and could be applied to determine the content of fleroxacin.

关 键 词：氟罗沙星 铬天青S 电荷转移化合物 卤键 

分 类 号：R927.2[医药卫生—药学]