姜黄素对实验性肝纤维化大鼠结缔组织生长因子表达的影响  

Effect of curcumin on expression of CTGF in experimental rats with hepatic fibrosis

作　　者：刘中禄[1] 孙仁山[2] 冉新泽[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,400042 [2]第三军医大学

出　　处：《实用医技杂志》2009年第2期85-86,共2页Journal of Practical Medical Techniques

摘　　要：目的观察中药姜黄素抗四氯化碳(CCl4)诱导大鼠肝纤维化的疗效及作用机制。方法将50只Wistar大鼠分为正常对照组、模型对照组、中药治疗1组及2组,于造模第1周及第4周开始用姜黄素治疗。免疫组织化学技术检测结缔组织生长因子(CTGF)在肝内表达及其在细胞中的定位含量。结果经中药姜黄素治疗后肝纤维化大鼠肝组织内CTGF表达降低,CTGF的免疫阳性反应信号主要位于纤维间隔中的细胞质。结论中药姜黄素能有效地减轻CCl4诱导肝纤维化大鼠的损伤及纤维化程度,其机制可能是抑制肝内CTGF表达。Objective To investigate t he effect and mechanism of Curcumin on liver fibrosis induced by exposure to carbontetrachloride （CCl4） in rats. Methods Fifty Wistar rat s were randomly divided into four groups： healthy controls group, CCl4 model group, Chinese herbs group 1, and group 2. Rats exposed to CCl4 were treated with Curcumin starting at the first and fourth week respectively after CCl4. The expression of connective tissue growth factor （CTGF） in liver tissues and their locations in cells were determined by immunohistochemical method. The liver histopathology was obsen, ed by an electron-microscope. Results The expression of CTGF was decreased in liver tissues of rat s exposed to CC14 after the treatment of Curcumin （P〈0.05）. The immuno-positive reaction signals were mainly expressed in cytoplasm of fiber septum and in liver cytoplasm. Conclusion The Chinese herbs Curcumin has protective effect against the liver injury and can inhibit liver fibrosis induced by CCl4 exposure in rats. The mechanisms may be consisted in its ability of down-regulating CTGF.

关 键 词：姜黄素 肝硬化 大鼠 胞间信号肽类和蛋白质类 

分 类 号：R285[医药卫生—中药学]