UVA与UVB对人体黑素细胞生长的影响  

Influence of UVA and UVB on Human Melanocytes Growth

作　　者：潘建英[1] 竺逸[1] 张玉彬[1] 沈光祖[1] 金泰廙[1] 金锡鹏[1] 洪琪(校对) 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]不详

出　　处：《环境与职业医学》2009年第1期43-45,共3页Journal of Environmental and Occupational Medicine

摘　　要：[目的]探讨长波紫外线（UVA）和中波紫外线（UVB）照射人体黑素细胞作用的差异。[方法]以不同剂量UVA和UVB分别照射黑素细胞，用四甲基偶氮唑盐微量酶反应比色法（MTT）测量细胞增殖率，比色法测定酪氨酸酶活性，吸光度测定黑素含量的变化。[结果]人体黑素细胞经0-2．25J／cm^2UVA照射后，黑素含量和酪氨酸酶活性无明显改变，其中较高剂量UVA（〉1．8J／cm^2）可使黑素细胞存活率下降。0-0．06J／cm^2UVB能明显抑制黑素细胞存活率，并能激活酪氨酸酶和促进黑素细胞的黑素合成。[结论]离体水平UVB对黑素细胞的促黑素合成作用明显强于UVA。[ Objective ] To investigate the distinct response between human melanocytes separately exposed to UVA and UVB. [ Methods ] The proliferation rate of melanocytes was determined by MTT assay. The viability of intracellular tyrosinase was determined by photoeolorimetry. The levels of intraeellular melanin was evaluated by spectrophotometry. [ Results ] Human normal melanocytes irradiated with 0-2.25 J/cm^2 UVA showed no marked changes while high dose UVA （〉1.8 J/cm^2） caused an obvious drop in cell proliferation. 0-0.06 J/cm^2 UVB showed significant increase in melanin content and tyrosinase activity and a drop of cell proliferation in dose-effect manner. [ Conclusion ] In vitro, the melanotropin synthesis of melanocytes induced by UVB was obviously greater than that by UVA.

关 键 词：长波紫外线 中波紫外线 黑素细胞 

分 类 号：R122.25[医药卫生—环境卫生学]