泡球蚴感染对BALB/c小鼠肝细胞ERK1/2和p38表达变化的初步研究  

Expression of ERK1/2 and p38 in Hepatic Tissue of BALB/c Mice Infected with Echinococcocus multilocularis

作　　者：纪静[1,2] 王俊华[1] 姜涛[1] 吕国栋[1] 刘辉[1] 李瑶[1] 王红丽[1] 卢晓梅[1] 王星[1] 段新宇[1] 温浩[1] 买买提祖农[2] 林仁勇[1] 

机构地区：[1]新疆医科大学第一附属医院新疆包虫病基础医学重点实验室,乌鲁木齐830054 [2]新疆医科大学基础医学院,乌鲁木齐830054

出　　处：《地方病通报》2009年第1期10-13,共4页Endemic Diseases Bulletin

基　　金：国家自然科学基金资助项目(30860253);新疆重点实验室开放课题基金资助项目(XJDX0202-2005-01;XJDX0202-2007-04)

摘　　要：目的通过检测ERK1/2和p38在泡球蚴感染小鼠肝组织中的表达,探讨其在肝泡球蚴病发生中的作用。方法采用半定量逆转录聚合酶链反应(RT-PCR)及免疫组织化学的方法检测16例感染组小鼠和16例对照组小鼠肝组织中的ERK1/2和p38的表达水平。结果感染组ERK1/2表达水平较对照组有统计学差异(P<0.05),p38的表达在感染组与对照组无统计学差异。结论在泡球蚴感染早期,肝细胞增殖占主导。Objective To investigate the expression of ERK1/2 and p38 in hepatic tissue of BALB/c mice infected with Echinococcocus multilocularis and molecular mechanism of Echinococcocus multilocularis. Methods Semi - quantitative reverse transcriptional polymerase chain reaction （ RT - PCR） and immunohistochemistry were employed to detect the expression level of ERK1/2 and p38 of 16 cases in the experimental group and 16 cases in control group. Results There was a statistically significant difference of ERK1/2 expression level between the experimental group and control group （ P 〈 0.05 ） , whereas there was no statistically significant difference of p38 expression. Conclusions The proliferation of hepatic cells has a closed relationship with the early infection of Echinococcocus multilocularis.

关 键 词：泡球蚴 ERK1/2 P38 小鼠 

分 类 号：R532.32[医药卫生—内科学] Q344.13[医药卫生—临床医学]