流式细胞术研究细胞凋亡的方法与技术  

Detecting Cell Apoptosis by Flow Cytometry Method

作　　者：潘春留[1,2] 闫雷[1] 娜仁图娜拉[1] 卿素珠[2] 关伟军[1] 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094 [2]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《现代生物医学进展》2009年第4期790-793,800,共5页Progress in Modern Biomedicine

基　　金：国家自然科技资源平台建设项目:2005DKA21101

摘　　要：细胞发生凋亡时,会伴随着一系列形态学、生物化学及分子生物学性质的变化,包括细胞皱缩,核染色质凝聚,细胞膜通透性改变,Caspases激活,线粒体跨膜电位降低,膜磷酯酰丝氨酸外化,胞质Ca2+浓度升高,DNA片段化及含量变化等特点。应用流式细胞术进行细胞凋亡的研究,对于探讨胚胎发育、衰老以及研究肿瘤的发生、发展和转化等病理生理过程和病毒感染及免疫等具有十分重要的意义。本文就细胞凋亡的特征、基于细胞膜功能的流式细胞术检测方法和基于细胞器功能的流式细胞术检测方法等关键性问题进行了阐述。During cell apoptosis, a series of morphological, biochemical and molecular changes happened including cellular shrinkage, permeability transition of plasma membrane, caspases activation, dissipation ofmitochondrial transmembrane potential, phos-phatidylserine redistribution, calcium flux, DNA fragmentation,Studying cell apoptosis with Flow cytometry has great meaning in the re- search of embryo growth, caducity and of the pathological process of tumor happening, development and transforming, It has been the hotspot in the life science field. Flow cytometry has the advantages of rapid, exact and multiparameter analysis of large populations of individual cells to investigate the biological processes associated with apoptosis. This paper focused on the principle and methods of flow cytometry detecting apoptosis.

关 键 词：流式细胞术 细胞凋亡 细胞膜研究方法 细胞器研究方法 

分 类 号：Q81[生物学—生物工程]