大鼠坐骨神经PDLLA/NGF套管模型脊髓背角c-fos表达的研究  

C-fos Expression in Spinal Dorsal Horn of Sciatic Nerve PDLLA/NGF Model Rats

作　　者：邬明[1] 唐忠志[1] 陈谦[1] 程青[1] 

机构地区：[1]广州军区武汉总医院急诊科,武汉430070

出　　处：《华南国防医学杂志》2009年第1期11-14,共4页Military Medical Journal of South China

基　　金：湖北省卫生厅科研基金资助项目(JX2B45)

摘　　要：目的建立大鼠外消旋聚乳酸/神经生长因子(PDLLA/NGF)复合套管模型,观察大鼠神经恢复过程中行为学改变和机械痛觉过敏的变化以及脊髓背角c-fos表达的改变,探讨外周神经损伤再生与痛觉过敏的关系。方法雄性Wistar大鼠40只,体重180～200g,随机分成2组,每组20只,A组为坐骨神经损伤PDLLA/NGF套管修复组;B组为假手术组。术后24日起检测行为学、机械刺激阈值、热缩足反射时间及c-fos表达计数。结果A组术后27天后机械痛阈和热缩足反射潜伏期与B组相差明显,有统计学意义。A组术侧L4～5脊髓背角与B组相比较,背角浅层c-fos计数增多,有统计学差异。A组c-fos、机械痛阈和热缩足反射潜伏期45日与27日均有统计学差异。结论大鼠PDLLA/NGF复合套管模型再生神经生长进入去神经支配区域后,触觉诱发疼痛和脊髓背角浅层c-fos表达,同时出现再生神经支配区域的痛觉过敏和疼痛相关行为。Objective To establish the sciatic nerve poly D, L-lactic acid/nerve growth factor （PDLLA/NGF） model for rats, to observe the behavioral response to tactile stimuli and changes of c-los expression in spinal dorsal horn during nerve recovery process, and to investigate the relationship between peripheral nerve injury regeneration and hyper- algesia. Methods 40 male Wistar rats were randomly divided into PDLLA/NGF group （group A） and sham-operation group （group B）. Under Fluothane anesthesia the right sciatic nerve of group A was isolated, 10mm of nerve defect was kept, and then repaired with PDLLA/NGF compound. For group B the right sciatic nerve was just exposed for 5min. Mechanic withdrawal threshold and thermal withdrawal latency were assessed by von-fray filaments and Hargreaves meth- od respectively 24, 27, 30, 33, 36, 39, 42, 45 days after operation. Five rats of each group were given innoxious touch- stimulus for 2 hours before killed at 27, 33, 39, 45 days after operation. Their spinal cord was obtained. Expression of c- los was investigated by Immunohistoehemistry. Results For group A the hyperalgesia appeared on the 24th day and reached the maximal on the 27th day after injury-operation. The threshold decreased severely at the 27th day and remained low for 2 weeks,then gradually increased. At the 45th day, the threshold was still significantly different from the sham operated animals. Meanwhile c-los expression elicited by touch-stimulus increased significantly and mainly distributed at middle of superficial layer in laminal Ⅰ-Ⅱ in spinal dorsal horn. The expression of c-fos decreased significantly between the 27th and 45th day in group A （P〈0. 05） and had significant difference between the two groups. The mechanic withdrawal threshold and thermal withdrawal latency significantly decreased between the 21st and 45th day in group A （P〈0. 05）. Conclusion For PDLLA/NGF model rats, once the regenerated nerve grows into the denervated skin, touch stimuli can induce pain-related beha

关 键 词：坐骨神经损伤 神经再生 神经性疼痛 痛觉过敏 C-FOS PDLLA/NGF复合套管 

分 类 号：R651[医药卫生—外科学]