平消胶囊含药血清对胆管癌细胞黏弹性和黏附力学特性的影响  

Effects of CANELIM Capsule Drug-contained Serum on Viscoelasticity and Adhesive Mechanical Properties of Cholangiocarcinoma Cells

作　　者：周大祥[1,2] 吴云鹏[3] 徐世荣[3] 

机构地区：[1]重庆三峡学院生物系 [2]三峡库区可持续发展研究中心,重庆404000 [3]重庆大学生物工程学院教育部生物力学及组织工程重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2009年第1期93-96,共4页Journal of Biomedical Engineering

摘　　要：将平消胶囊灌喂大鼠,制备含药血清,血清培养胆管癌细胞,采用微管吸吮技术,研究不同剂量的含药血清对胆管癌细胞黏弹性以及黏附力学特性的影响。结果显示,胆管癌细胞具有高弹性系数和低黏性系数的黏弹性特征,高剂量和中剂量含药血清处理后的胆管癌细胞黏弹性参数K1、K2和μ值较对照组均明显降低(P<0.01),低剂量变化不明显;细胞与CD44v6单克隆抗体的黏附力随着药物剂量的增加而显著降低(P<0.01)。以上结果提示平消胶囊破坏了胆管癌细胞骨架,抑制细胞膜上黏附分子CD44v6表达,降低癌细胞与血管内皮细胞的黏附几率,阻止胆管癌细胞转移。By way of gastrogavage, we administered CANELIM capsules to rat for prepering the drug-contained serums. And then the serums obtained were used to plant cholangioearcinoma cells. Lastly, using the micropipette aspiration technique, we investigated the effects which the drug-contained serums of different doses have on the vis- coelasticity and adhesive mechanical properties of cholangiocarcinoma cells. The results showed that cholangioearci- noma cells presented a characteristic of high elastic coefficient and low viscous coefficient. After being treated by the high dose and middle dose drug-contained serums, the viscoelastical properties of cholangiocarcinoma cells K1, K2 and μ evidently decreased （P〈0.01）. But the properties of low dose did not evidently change. The adhesive force between cholangiocarcinoma cells and CD44v6 protein significantly reduced with the increasing of the dose of CANELIM capsules（P〈0.01）. It is suggested that CANELIM capsules would destroy the cytoskeleton of cholan- giocarcinoma cells, restrain the adhesion molecule CD44v6 on membrane from expressing, reduce the adhesion probability between cholangiocarcinoma cells and vasal endothelial cells, and finally, prevent the metastasis of cholangiocarcinoma cells.

关 键 词：胆管癌细胞 平消胶囊 微管吸吮技术 黏弹性 黏附CD44v6 

分 类 号：R285.5[医药卫生—中药学] R735.8[医药卫生—中医学]