复乳法制备载牛血红蛋白纳米微球型血液代用品中表面活性剂、溶剂对包封率和粒径的影响  

Effects of Surfactant and Solvent on the Encapsulation Efficiency And Size in Using Double Emulsion Method for Preparing Bovine Hemoglobin Loaded Nanoparticles as Blood Substitutes

作　　者：章晓兰[1,2] 袁媛[1] 单晓茜[1] 盛燕[1] 徐锋[1] 刘昌胜[1] 

机构地区：[1]华东理工大学,教育部医用生物材料工程研究中心,上海200237 [2]上海市科技工程学院,上海200231

出　　处：《生物医学工程学杂志》2009年第1期116-121,共6页Journal of Biomedical Engineering

基　　金：十五“863”纳米专项基金资助项目(2004AA-302050);上海市科委纳米专项基金资助项目(0452nm022)

摘　　要：在前期研究基础上,采用复乳化法制备载牛血红蛋白纳米微球型血液代用品。免去传统的匀浆强力分散措施,通过表面活性剂的加入及溶剂筛选,在初乳和复乳步骤均采用较温和的超声波分散,获得97%以上的高包封率、平均粒径约286 nm微球。油相中表面活性剂亲水-亲油平衡值对包封率有显著影响,且与外水相表面活性剂匹配才能获得高的包封率。混合表面活性剂Poloxamer188/Span80比单一表面活性剂Span80能更稳定乳液,提高包封率。表面活性剂浓度高,有利于获得高的包封率和窄的粒径分布。但超过一定量时,对包封率影响不明显,而粒径及粒径分散指数增大。溶剂脱除速率适中有利于获得较高的包封率、较小的粒径和粒径分散指数。On the basis of previous researches, we have prepared Bovine hemoglobin-loaded nanoparticles （HbP）, using the double emulsion method. More mild dispersing treatment was employed during the primary and secondary emulsion; over 97% encapsulation efficiency （EE%） and an average size about 286 nm were achieved by u- sing surfactants, screening solvents, as well as avoiding the traditional strong dispersing process. The value of Hy- drophile-lipophile balance in oil phase exerted a significant effect on EE%and led to higher EE~ when matched with the surfaetants in outer aqueous phase. When compared with the sole solvent Span80, the mixed surfactants such as Poloxemer188/Span80 stabilized the emulsion more efficiently and increased the EEl. The higher concentration of surfactants resulted in higher EE% and narrower size distribution. But over some amount, the surfactants had no significant effect on EE%, resulting in larger size and polydispersity index （PDI）. The appropriate removal rate of solvents contributes to higher EE%, smaller size and PDI.

关 键 词：表面活性剂 溶剂 包封率 粒径 

分 类 号：R318.08[医药卫生—生物医学工程]