人牙胚组织在裸鼠肾囊膜培养模式下的成牙能力  

Odontogenetic capacity of human tooth germ in kidney capsule culture mode

作　　者：胡雪峰[1,2] 胡晓笑[3] 

机构地区：[1]福建师范大学生命科学学院,福建省福州市350007 [2]发育与神经生物学福建省高校重点实验室,福建省福州市350007 [3]福建师范大学福清分校生物与化学工程系

出　　处：《中国组织工程研究与临床康复》2009年第7期1224-1226,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：福建省教育厅项目资助(JB06088)~~

摘　　要：背景:建立一种科学可靠、简便可行,既可以人为进行干预,又不会影响牙齿继续发育的体外培养模式对于了解人类牙齿发育的组织学特点至关重要。目的:将流产人胚胎12周的磨牙牙胚植入到裸鼠肾囊膜下培养,观察其成牙情况,建立人牙胚再生的培养模式。设计、时间及地点:细胞水平的动态观察实验,于2005-03/2006-12在福建师范大学生命科学学院和发育与神经生物学福建省高校重点实验室完成。材料:12周流产人胚胎由福州市第二人民医院妇产科提供,6～10周龄Blab/c裸鼠。方法:无菌条件下分离新鲜的12周流产人胚胎下颌,分离磨牙牙胚。将牙胚移植到裸鼠肾囊膜下分别培养4,8,12和16周,取移植块。主要观察指标:以苏木精-伊红染色和Azon法染色后进行形态学观察。结果:生长4周的人牙胚内,可以观察到造釉器和牙乳头的细胞发生形态改变,牙上皮细胞和间充质细胞开始极化,胞体变长形成前成釉质细胞和前成牙本质细胞。生长8周时,前成釉质细胞和前牙本质细胞的极化变得更加明显,细胞已变成高柱状,在未来牙尖区域可发现少量牙本质的形成。培养12周时,可见连续完整的牙本质。生长16周的人牙胚,已经形成连续完整的成釉质细胞,牙釉质、牙本质和成牙本质细胞层。结论:肾囊膜是人牙胚组织体外培养的良好模式。BACKGROUND： It is important to establish a reliable, convenient, feasible and regulated method to culture tooth in vitro. So we can learn more about human tooth development in the histological feature. OBJECTIVE： The molar tooth germ of 12 weeks abortion human embryo was transplanted and cultured under kidney capsule to see the dentiparous level and establish the culture mode of the human tooth germ regeneration. DESIGN, TIME AND SETTING： Observational comparative study was performed in the College of Life Sciences, Fujian Normal University and State Key Laboratory of Developmental Biology and Neurobiology from March 2005 to December 2006. MATERIALS： Human abortion embryo of 12 weeks was offered by the Department of Gynaecology and Oobstetrics, the Second People＇s Hospital of Fuzhou. Blab/c nude mice of 6-10 weeks old were also used. METHODS： Fresh lower mandible of 12 weeks abortion human embryo was separated; human molar tooth germ was isolated. The tooth germ was transplanted under nude mice kidney capsule and cultured for 4, 8, 12 and 16 weeks, respectively. Then the graft was taken out for further use. MAIN OUTCOME MEASURES： Hematoxylin-eosin staining and Azon staining were used for morphological observation. RESULTS： In the 4 weeks growth of human tooth germ, morphological changes could be seen in the enamel organ and dental papilla cells, dental epithelium cells and mesenchymal cells began polarized, cell lengthened to be pre-ameloblast and pre-odontoblast. After 8 weeks growth, the polarization became more obvious in the pre-ameloblast and pre-odontoblast. Cells became high stylolitic shape and small amounts of dentin formation were found in the future of dental cusp region. After 12 weeks growth, continuous and intact dentin was found. After 16 weeks growth, continuous and intact ameloblast, enamel, dentin, odontoblast was found. CONCLUSION： Kidney capsule is a good mode for in vitro culture of human tooth germ.

关 键 词：牙胚 器官培养技术 牙齿生长 肾囊膜 

分 类 号：R329.33[医药卫生—人体解剖和组织胚胎学]