流行性乙型脑炎病毒脑脊液分离株“47株”全基因组特征分析  

Molecular characterization of fuli-length genome of Japanese encephalitis virus strain 47 isolated from patient＇s cerebrospinal fluid in China

作　　者：潘晓玲[1] 王环宇[1] 付士红[1] 韩娜[2] 刘海舟[2] Simon Rayner 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒性脑炎室,北京100052 [2]中国科学院武汉病毒所生物信息部

出　　处：《中华实验和临床病毒学杂志》2009年第1期26-28,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的对1950年分离自我国黑龙江省患者脑脊液的乙脑病毒“47株”进行全基因组序列的测定和分析，全面了解其全基因组特征。方法复苏毒种提取病毒RNA，使用自行设计的乙脑病毒全基因组扩增测序引物，完成对病毒全基因组序列的测定。采用DNAStar、Modehest、Phylip等生物软件完成全基因组核苷酸、氨基酸序列差异分析和乙脑病毒全基因组的系统进化分析。结果乙脑病毒“47株”全长10977个核苷酸。96至10391位为开放读码框ORF，共10296个核苷酸，编码3432个氨基酸。“47株”与5株疫苗株在全基因组水平的核苷酸差异在2．4％-4．4％之间，氨基酸差异在0．3％～1．1％之间。乙脑病毒全基因组最适进化模型为GTR＋I＋G。全基因组进化分析显示“47株”属于基因Ⅲ型乙脑病毒。结论“47株”全基因组核苷酸和氨基酸高度保守，属于基因Ⅲ型乙脑病毒。Objective To sequence and analyze the whole genome of Japanese encephalitis virus （JEV） strain named 47 which was isolated from patient＇s cerebrospinal fluid sample in Heilongjiang province in 1950. Methods RNA was extracted from the recovery strain 47 and amplified with self-designed JEV genome sequencing primers. The differentiation analysis for nucleotides and coding amino acids and phylogenetic analysis were performed by the software of DNAStar, Modeltest, and Phylip. Results The whole genome of strain 47 has 10 977 nucleotides. An open reading frame from 95 to 10 391 including 10 296 nucleotides is capable of coding a 3432 amino acid polyprotein. The nucleotide difference between strain 47 and 5 vaccine strains is 2.4 %-4.4 % , the amino acid difference between strain 47 and 5 vaccine strains is 0.3 %-1.1%. The best evolution model for the whole genome is GTR ＋ I ＋ G. Based on the phylogenetic analysis, strain 47 belongs to the genotype Ⅲ JEV. Conclusion Strain 47 is highly conserved on whole genome nucleotide and amino acid sequence. And it is belongs to the genotype Ⅲ JEV.

关 键 词：脑炎病毒 日本 种系发生 基因扩增 序列分析 

分 类 号：R51[医药卫生—内科学]