应用生物素-链霉亲和素酶联免疫吸附法检测原发性肝癌特异性γ-谷氨酰转移酶  

Clinical application of avidin-biotin ELISA to detect serum hepatoma-specific γ-glutamyltransferase in patients with primary hepatic cancer

作　　者：王念跃[1] 赵伟[1] 张岱[2] 张永臣 端木浩[1] 

机构地区：[1]东南大学医学院附属南京第二医院肝病检测中心,210003 [2]江苏大学医学技术学院检验系

出　　处：《中华肿瘤杂志》2009年第2期114-117,共4页Chinese Journal of Oncology

基　　金：南京市医学重点科技发展项目（ZKX0303）

摘　　要：目的观察生物素-链霉亲和素酶联免疫吸附（ELISA）法检测原发性肝癌（PHC）特异性欧曼陀罗凝集素强结合的γ-谷氨酰转移酶（DSA-GGT）的临床应用价值。方法对45例正常对照者、58例PHC患者和203例非PHC患者（包括其他肿瘤患者36例，肝良性病变患者167例），应用生物素-链霉亲和素ELISA法检测受试者血清中DSA—GGT的水平，同时用ELISA法检测受试者血清中甲胎蛋白（AFP）的水平。结果58例PHC患者中，DSA—GGT阳性38例，检测DSA-GGT诊断PHC的灵敏度为65．5％；203例非PHC患者中，DSA—GGT阳性18例，检测DSA—GGT诊断PHC的特异度为91．1％。该方法的平均批内及批问变异分别为8．9％和11．5％。58例PHC患者中，AFP阳性40例，检测AFP诊断PHC的灵敏度为69．0％；203例非PHC患者中，AFP阳性19例，检测AFP诊断PHC的特异度为90．6％。DSA-GGT与AFP联合检测诊断PHC的灵敏度和特异度分别为93．1％和85．7％。结论生物素-链霉亲和素ELISA法检测DSA—GGT和ELISA法检测AFP对PHC诊断的灵敏度和特异度相近，且前者的重复性良好，可作为PHC诊断的较好检测方法；DSA—GGT与AFP联合检测可显著提高PHC诊断的灵敏度。Objective To detect serum hepatoma-specific datura stramonium lectin-tightly binding γ-glutamyl transferase （DSA-GGT） in patients with primary hepatic cancer （PHC） by avidin-biotin ELISA method which was established in our laboratory, and carry on a study of its clinical application. Methods To detect serum DSA-GGT in 45 healthy control subjects, 58 PHC patients and 203 non-PHC patients （including 36 patients with other tumors and 167 patients with benign liver diseases） with the method was established; meanwhile, AFP was detected by ELISA method. Results 38 individuals were DSA-GGT positive in 58 PHC patients, the sensitivity was 65.5%. 18 individuals were DSA-GGT positive in 203 patients without PHC, the specificity was 91.1%. The sensitivity and specificity of AFP in diagnosis of PHC patients was 69.0% and 90.6% , respectively. The sensitivity and specificity of combination of DSA-GGT and AFP was 93. 1% and 85.7% , respectively. The average intra-CV and inter-CV of DSA-GGT ELISA was 8.9% and 11.5% , respectively. Conclusion The sensitivity and specificity of DSA-GGT ELISA method established in our lab is similar with that of AFP assay and the accuracy is good. Combination of DSA-GGT and AFP may improve the diagnostic sensitivity. The method should be potentially as a new way to improve diagnosis of PHC.

关 键 词：生物素-链霉亲和素 Γ-谷氨酰转移酶 肝肿瘤 酶联免疫吸附试验 

分 类 号：R686[医药卫生—骨科学]