人精浆中A型血型物质的分离纯化及理化性质研究  

Studies of the isolation, purification and physico-chemical characterization of human saminal plasma blood groop substance A(spbgs-A)

作　　者：王保捷[1] 李春梅[1] 李庆生[1] 肖仰哲[1] 张核子[1] 卢岩[1] 

机构地区：[1]中国医科大学法医学系,沈阳110001

出　　处：《中国法医学杂志》1998年第1期25-27,共3页Chinese Journal of Forensic Medicine

基　　金：国家教委归国人员基金;辽宁省博士启动基金

摘　　要：应用凝胶过滤、阴离子高速液相色谱及免疫亲合层析方法成功地从A型分泌型人精浆中分离、纯化了具有A型活性的精浆血型物质（SPBGS－A）。理化分析结果表明：SPBGS－A为表现单一A型活性、分子量不均一的糖蛋白。其分子量约为100KD；等电点在pH2．7～3．5之间；糖含量占72．5％；蛋白质含量占24．2％。共检出了16种氨基酸成分，其中苏氨酸、丝氨酸及脯氨酸的含量约占50％。经与其它水溶性血型物质在化学组成上进行比较，提示其可能具有一定的器官特异性。Active SPBGS-A was successfully isolated and purified from seminal plasma of A secretory type individuals by procedures involving gel filtration,anion exchange colqmn chromatography with FPLC system and immunoaffinity chromatography. Physico-chemical analysis suggested that SPBGS-A showed only A cativity,and was a kind of glycoproteins of high heterogeneity. Molecular weight of SPBGS-A was approximately 50 to 70KD and isoelectric point was 2. 7 to 3. 5. It contained nealy 72. 5 % of carbohydrate and 24. 2 % of protein in which Thr, Ser and Pro amouted to 50% of total 16 kinds of amino acid. The result of comparison between it and other water-soluble blood group substances indicated that SPBGS-A might possess organ specificity.

关 键 词：精浆 血型物质 纯化 器官特异性 

分 类 号：D919.2[医药卫生—法医学]