血管紧张素-Ⅱ对血管内皮细胞游离钙离子浓度的影响及阿托伐他汀的保护作用  

Effect of Ang-Ⅱ on intracellular free calcium concentration in vascular endothelial cells and the protective effect of atorvastatin

作　　者：韩磊[1] 李鸣皋[2] 叶平[1] 

机构地区：[1]解放军总医院老年心血管二科,北京市100853 [2]海军总医院航空潜水医学中心,北京市100037

出　　处：《实用医学杂志》2009年第4期511-512,共2页The Journal of Practical Medicine

基　　金：海军后勤科研基金资助项目(编号:00-3321)

摘　　要：目的:观察血管紧张素-Ⅱ(Ang-Ⅱ)对血管内皮细胞(VEC)游离钙离子浓度([Ca2+]i)的影响及阿托伐他汀的保护作用。方法:将血管内皮细胞分为空白对照组(仅给予细胞培养液)、Ang-Ⅱ组(细胞培养液中加入Ang-Ⅱ,使其终浓度为10-7mol/L)、Ang-Ⅱ+小剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为0.1μmol/L)、Ang-Ⅱ+大剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为1μmol/L)。用激光共聚焦显微镜测量各组细胞的[Ca2+]i。结果:(1)Ang-Ⅱ组的[Ca2+]i显著高于空白对照组(P<0.01);(2)Ang-Ⅱ+阿托伐他汀组的[Ca2+]i显著低于Ang-Ⅱ组(P<0.01)。(3)Ang-Ⅱ+大剂量阿托伐他汀组的[Ca2+]i低于Ang-Ⅱ+小剂量阿托伐他汀组(P<0.05)。结论:Ang-Ⅱ可引起细胞内[Ca2+]i的显著增加,阿托伐他汀对Ang-Ⅱ所致的[Ca2+]i增加具有保护作用,且这一作用呈剂量依赖性。Objective To investigate the effect of Ang-Ⅱ on intracellular free calcium concentration（[Ca^2＋]i） in vascular endothelial cells （VEC） and the protective effect of atorvastatin. Methods VEC were divided into 4 groups： control group （only given cell culture medium）; Ang-Ⅱ group （10^-7 mol/L Ang-Ⅱ ）; Ang-Ⅱ ＋ low dose atorvastatin group （0.1 μmol/L atorvastatin, 10^-7 mol/L Ang-Ⅱ ） ; Ang-Ⅱ ＋ high dose atorvastatin group （1 μmol/L atorvastatin, 10^-7 mol/L Ang-Ⅱ ）. [Ca^2＋]i were measured by laser scanning confocal microscopy in the four groups. Results [Ca^2＋]i in Ang-Ⅱ group was significantly higher than those in control group （P 〈 0.01 ）, Ang-Ⅱ ＋ low dose atorvastatin group （P 〈 0.01）, and Ang-Ⅱ ＋ high dose atorvastatin group （P 〈 0.01 ）. [Ca^2＋]i in Ang-Ⅱ ＋ high dose atorvastatin group was significantly lower than that in Ang-Ⅱ ＋ low dosage group（P 〈 0.05）. Conclusion Ang-Ⅱ can increase [Ca^2＋]i in VEC, which can be pretected by atorvastatin in a dose-dependent manner.

关 键 词：血管紧张素Ⅱ 内皮细胞 CA2+ 阿托伐他汀 

分 类 号：R96[医药卫生—药理学]