偏凸山羊草和硬粒小麦远缘杂交后代高分子量麦谷蛋白亚基分析  

Composition Analysis of High-molecular-weight Subunits of Glutenin of the Progenies Derived from Aegilops ventricosa×Durum Wheat

作　　者：李鹏[1] 张素勤[1] 郑竹[1] 李松桃[1] 张庆勤[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《西北农业学报》2009年第1期48-51,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：教育部春晖计划(15001D-3);贵州省优秀科技教育人才省长基金(黔省专合字(2007)19;2007~2010);贵州省科技支撑农业攻关计划项目(黔科合NY字[2008]3017号;2008~2012)

摘　　要：以偏凸山羊草和硬粒小麦(Sauwne20)远缘杂交F6代的30个株系为试材,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法鉴定分析了杂交后代高分子量麦谷蛋白亚基组成。结果表明:F6株系Glu-1位点变异较大,出现了17种亚基类型。在杂交后代中出现了1、2*、13+16、5+10等优质亚基,其中1亚基频率高达77.4%;5+10亚基频率高达74.2%;同时还出现了7、6+8、7+8+21、13+16+21等稀有亚基。64.8%株系的高分子量麦谷蛋白亚基品质评分高达10分。The compositions of high-molecular-weight （HMW） subunits of glutenin of the F6 progenies derived from Aegilops ventricosa × durum wheat （cultivar ‘Sauwne20＇） were researched using sodium dodecyl sulphate-polyacrylamide gel electrophoresis （SDS-PAGE） method in the present study. The results showed that high variations were found in the loci of Glu-1 of F6 lines, and 17 types of HMW-GS appeared. High quality subunits （subunit pairs） appeared such as 1,2 ＊,7＋8, 13＋16,5＋ 10, and so on, in which the frequency of subunit 1 was as high as 77.4% and the frequency of subunit pair 5＋10 was as high as 74.2%; in addition, some infrequent subunit pairs 7, 6＋8, 7＋8＋21 and 13＋16＋21 occurred 64.8% of lines scored up to 10.

关 键 词：偏凸山羊草 硬粒小麦 远缘杂交 高分子量麦谷蛋白亚基 品质育种 

分 类 号：S512.1[农业科学—作物学]