顶空气相色谱法测定湖北贝母提取物中的有机溶剂残留量  

Determination of organic residues in Fritillaria hupehensis extracts by capillary gas chromatography with headspace sampling

作　　者：张鹏[1] 周伟 陈霞[1] 皮慧芳[1] 阮汉利[1] 吴继洲[1] 

机构地区：[1]华中科技大学同济药学院,天然药物化学与资源评价湖北省重点实验室,湖北武汉430030 [2]湖北省药品检验所,湖北武汉430064

出　　处：《中国医院药学杂志》2009年第4期259-262,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家中医药管理局新药开发专项课题(编号:D2X004A)

摘　　要：目的:建立湖北贝母提取物中D101型大孔吸附树脂有机溶剂残留量的检测方法。方法:采用顶空气相色谱法,以HP-1(30m×0.25mm,0.25μm)毛细管柱分离、氢火焰离子化检测器检测。结果:6种大孔吸附树脂残留溶剂在16min内能很好的分离;该方法的精密度RSD均小于6.0%;加样回收率为90.8%～105.8%;峰面积与浓度均有良好的线性关系。结论:该方法操作简便、准确可靠,可控制湖北贝母提取物中D101型大孔树脂有机溶剂残留量。OBJECTIVE To establish a method for determination of the organic solvent residues including n hexane, benzene, methylbenzene, ethyl benzene, xylene, styrene, divinylbenzene from D101 macroporous adsorption resin in Fritillaria hupehensis Hsiao et K. C. Hsia extracts. METHOD6 The residues from D101 macroporous adsorption resin were analyzed by headspace gas chromatography using HP-1 （30 m× 0. 25 mm, 0. 25μm）capillary column and a FID. RESULTS ganic residues were well separated in 16 minutes. RSD of precision were all less than 6. 0%. The recoveries of 90. 82% - 105.83%. There was favorable linearity relation between peak area and concentration. CONCLUSION is sensitive, accurate and can be used for quality control. All the 6 or organics were The method

关 键 词：湖北贝母 提取物 大孔吸附树脂 有机溶剂残留量 顶空气相色谱法 

分 类 号：R917[医药卫生—药物分析学]