反相高效液相色谱法测定PEG胁迫下小麦幼苗中甜菜碱的含量变化  

The determination of Betaines in wheat seedings under drought stress by RP-HPLC

作　　者：马钰[1] 蒋斌[1] 王庆俐[1] 张林生[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2009年第1期190-194,共5页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金资助项目(30671253);中国博士后科学基金资助项目

摘　　要：采用胁迫及复水处理不同品种小麦幼苗,甜菜碱提取液经浓缩后分别在阴阳离子交换树脂上纯化,样品用C18反相色谱柱进行分离,流动相为50 mmol/L的NaH2PO4溶液(pH4.45),流速为0.6 mL/min,192 nm波长测定。实验优化了提取温度、时间、色谱离子对等相关影响因素,测定了水分胁迫下小麦幼苗叶片中甜菜碱含量的变化。研究结果发现:在PEG胁迫和复水过程中,随时间的延长,小麦幼苗中的甜菜碱的含量均呈现先增后降趋势,抗旱型小麦能在较短时间内迅速积累大量甜菜碱;甜菜碱的合成与植物抗旱有着密切关系。反相高效液相色谱法测定小麦甜菜碱含量具有准确、灵敏、简便等特点。We used the different wheat seedings which were planted on the condition that water is restricted and resupply. The concentrated betain samples were purified by passing through the ion-exchange resin. We used the Reverse-Phase High Performance Liquid Chromatography technology to determine the change of betaine content. In the HPLC system, the buffer is 0.05 M sodium phosphate（pH4.45）, the column was kept at 28℃ and the flow rate was 0.6 mL/min, the samples was determined at 192 nm wavelength. Besides, we optimized the factors, such as temperature, time and ion pair. During both the PEG- induced drought stress and water resupplied process, the amount of betaine increased at first and reduced latter. A great deal of Betaine accumulated in the anti-drought type wheat in a short time. This result showed that the synthesis of betaine have close connection with anti-drought plant. This method was exact, sensitive and convenient, as well provided a basis for anti-drought mechanism and the wheat breeding work.

关 键 词：小麦幼苗 水分胁迫 甜菜碱 反相高效液相色谱法 

分 类 号：S512.101[农业科学—作物学]