青海渔水河嗜盐耐盐放线菌的初步研究  

The preliminary investigation of halophilic and halotolerant Actinomycetes in Yushuihe,Qinghai

作　　者：朱华[1] 来航线[2] 傅艳萍[1] 薛泉宏[2] 孙婷[2] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2009年第1期233-236,共4页Agricultural Research in the Arid Areas

摘　　要：对青海渔水河河水、底泥及河旁沙土进行盐分离子及pH值的测定,并通过不同培养基、盐种类和添加量的设计对渔水河中嗜盐耐盐放线菌资源进行了初步研究。结果表明:渔水河样品均偏碱性;河水的水溶性总盐及盐分离子含量均远远低于沙土,其中泥土的水溶性总盐含量最高,为158.67 g/kg;盐分阴离子中Cl-含量最高,阳离子中K+和Na+含量最高,所以推断样品盐分离子主要以NaCl和KCl的形式存在;分离共获得89株放线菌,其中底泥中分离到的放线菌最多,且改良淀粉酪素培养基的分离效果最好;通过嗜盐耐盐放线菌的筛选,得到8株嗜盐放线菌,其中极端嗜NaCl菌3株,极端嗜KCl菌7株,还有1株嗜K型放线菌。Salt ions and pH value of river water, sediment and sandy soil of Yushuihe were measured in this paper, and some preliminary research on halophilic and halotolerant Actinomycetes have been done through design different media, tapy of salt and addition quantity. The results showed that： all the experimental samples are alkaline; the the highest total content of water-soluble salt is sediment which is 158.67 g/kg, then is sandy soil- 90.43 g/kg, river water is the lowest one which is 3.1 g/L; as for salt ions, the highest anion content is Cl-, while among the cations, K＋ and Na~ are much higher. Therefore, NaCl and KCl are the main existing forms of salt ions in the samples; 89 strains of Actinomycetes were obtained and most of them were separated from sediment, and the best isolation medium is modified starch casein medium; 8 halophilic Actinomycetes strains were discovered by halophilic and halotolerant Actinomycetes screening, and 3 of them are extreme halophilic Actinomycetes in NaCl while 7 in KCl, and 1 of them is tropic - K Actinomycetes.

关 键 词：渔水河 盐分离子 放线菌 嗜盐放线菌 筛选 

分 类 号：Q938.2[生物学—微生物学]