HPLC测定血清中阿昔洛韦浓度的方法改进  

An Improved HPLC Method for the Determination of Serum Acyclovir Concentration

作　　者：张宏伟[1] 潘家祜[1] 吴超[1] 戴晓华 李端[1] 

机构地区：[1]上海医科大学药学院药理教研室

出　　处：《药物分析杂志》1998年第2期90-92,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：改良ＨＰＬＣ测定人及大鼠血清中阿昔洛韦浓度的分析方法．使用Ｗａｔｅｒｓ高效液相色谱仪，μ－ＢｏｎｄａｐａｋＣ１８柱，流动相为甲醇—水（３９７，Ｖ／Ｖ），流速１．５ｍＬ／ｍｉｎ，检测波长２５４ｎｍ，内标物为扑热息痛，血清样品采用甲醇去蛋白．阿昔洛韦血清浓度在０．０５～１．６ｍｇ／Ｌ范围内与峰高呈良好线性关系，大鼠和人血清中相对回收率分别为９３．３１％及９０．６９％；最低检测浓度均为０．０２５ｍｇ／Ｌ；天内精密度分别为０．８３％～２．００％及１．１０％～２．５８％；天间精密度分别为２．０１％～５．９０％及３．９０％～６．２７％．An improved HPLC method for the determination of Acyclovir(Acy)in serum was developed.A Waters HPLC instrument with μ-Bondapak C18 column was used.The mobile phase was a mixture of methanol and water in a ratio of 3∶97(V/V)at a flow rate of 15mL/min,the detector was set at 254nm.Acetaminophen was used as internal standard.The serum sample was extracted by a methanol protein precipitation method.Serum concentration of Acy ranging from 005mg/L to 16mg/L showed good linear relationship with the peak ratio and the minimum concentration detected was 0025mg/L.The average recoveries in rat and human were 9331% and 9069% respectively.The intraday precision was <3% and interday precision was <7%.

关 键 词：阿昔洛韦 HPLC 血药浓度 

分 类 号：R978.7[医药卫生—药品]