干旱对麻疯树部分生理生化指标及基因表达的影响  

Effect of drought stress on physiological and biochemical indexes and gene expression of Jatropha curcas

作　　者：黄静[1] 戴晓[1] 彭书明[1] 徐莺[1] 陈放[1] 魏炜[1] 

机构地区：[1]生物资源与生态环境教育部重点实验室,四川省生物资源与生物制药重点实验室四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2009年第1期223-227,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家“十五”科技攻关(2002BA901A15);国际科技合作计划项目(2003DFB00005,2004DFB00300)

摘　　要：用30%的聚乙二醇(PEG-6000),模拟干旱逆境对麻疯树幼苗进行干旱胁迫,分别测定不同胁迫程度下麻疯树幼苗中水势、可溶性糖、脯氨酸、甜菜碱、丙二醛等部分生理生化指标的变化,以研究麻疯树的抗旱机理。结果表明,麻疯树幼苗对干旱胁迫较为敏感,随胁迫程度的加深,叶片水势下降,可溶性糖、脯氨酸、甜菜碱、丙二醛含量均呈现出一定的变化规律,初步说明麻疯树在遭受干旱胁迫时其渗透调节作用会增强,生物膜的通透性也会增加。同时,通过mRNA差异显示技术(DDRT-PCR)研究发现对照组和干旱胁迫组的基因表达存在着明显的差异,说明干旱可以诱导麻疯树的某些基因表达。因此麻疯树在遭受干旱胁迫时可以在多个层面上发生响应机制以抵抗逆境对其造成的伤害。The drought-resistance ability of Jatropha curcas seedlings were studied with the treatment of 30% poly-ethylene glycol （PEG-6000）. Indicators such as relative water content （RWC） and soluble suger as well as proline, betaine and malondialdehyde （MDA） etc. were monitored for the duration of adverse environmental conditions of drought stress. The result showed that drought stress had significant influence on Jatropha curcas seedlings,such as decreased RWC in seedlings, with increased content of soluble suger, proline, betaine, MDA, indicating increased property of osmoregulation and permeability of bio - membrane . Meanwhile, using differential display reverse transcription-polymerase chain reaction （DDRT-PCR） methodology, several differentially expressed genes of Jatropha curcas were identified, which indicated that there were several physiological responses of Jatropha curcas to adverse environmental conditions.

关 键 词：麻疯树 生理生化指标 干旱胁迫 

分 类 号：Q945[生物学—植物学]